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Using Fluorescence In Situ Hybridization (FISH) Analysis to Measure Chromosome Instability and Mosaic Aneuploidy in Neurodegenerative Diseases

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Genomic Mosaicism in Neurons and Other Cell Types

Part of the book series: Neuromethods ((NM,volume 131))

Abstract

Chromosome instability is a form of genomic instability that leads to cells with an abnormal number of chromosomes, defined as aneuploidy. Aneuploidy that results from chromosome instability can be complete or mosaic, depending on whether all or only some of the cells that make up an organism have an abnormal number of chromosomes. Aneuploidy is associated with many human conditions, such as cancer and Down syndrome (DS, trisomy 21), and it has more recently become a focus of investigation in neurodegenerative diseases, including Alzheimer’s disease (AD), Niemann-Pick C1 (NPC), and frontotemporal lobar degeneration (FTLD). In these disorders, aneuploid cells in affected brain regions appear to contribute significantly to apoptosis and neurodegeneration, and may thus underlie the associated cognitive deficits. Herein, we describe the methods that our laboratory has developed to analyze the frequency of chromosome instability (i.e., mosaic aneuploidy) in AD, NPC, and FTLD and associated cell death. Our goal is to provide the reader with guidelines for using these methods and to offer insights into their utility and potential limitations.

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Authors and Affiliations

  1. Department of Neurology, Rocky Mountain Alzheimer’s Disease Center, and Linda Crnic Institute for Down Syndrome, University of Colorado School of Medicine, 12700 E. 19th Ave. MS8608, Aurora, CO, 80045, USA

    Julbert Caneus, Antoneta Granic, Heidi J. Chial & Huntington Potter

  2. Neuroscience Program, University of Colorado, Anschutz Medical Campus, Aurora, CO, 80045, USA

    Julbert Caneus & Huntington Potter

  3. Institute of Neuroscience, Newcastle Institute for Ageing, Newcastle University, Newcastle upon Tyne, NE4 5PL, UK

    Antoneta Granic

  4. NIHR Newcastle Biomedical Research Centre in Ageing and Chronic Disease, Newcastle University, Newcastle upon Tyne, NE4 5PL, UK

    Antoneta Granic

  5. Newcastle upon Tyne NHS Foundation Trust, Campus for Ageing and Vitality, Newcastle University, Newcastle upon Tyne, NE4 5PL, UK

    Antoneta Granic

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  1. Julbert Caneus
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  2. Antoneta Granic
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  3. Heidi J. Chial
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  4. Huntington Potter
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Correspondence to Huntington Potter .

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Editors and Affiliations

  1. Department of Molecular, Cellular and Developmental Neurobiology, Cajal Institute (IC-CSIC), Madrid, Spain

    José María Frade

  2. Biological Studies and Laboratory of Genetics, The Salk Institute, La Jolla, California, USA

    Fred H. Gage

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Caneus, J., Granic, A., Chial, H.J., Potter, H. (2017). Using Fluorescence In Situ Hybridization (FISH) Analysis to Measure Chromosome Instability and Mosaic Aneuploidy in Neurodegenerative Diseases. In: Frade, J., Gage, F. (eds) Genomic Mosaicism in Neurons and Other Cell Types. Neuromethods, vol 131. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7280-7_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7280-7_16

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