Abstract
Long double-stranded (ds) RNA molecules are produced as a byproduct of viral replication. Studying virus-derived dsRNA is important for understanding virus replication, understanding host responses to virus infections, and as a diagnostic tool for virus presence and replication. Here, we describe four different techniques for visualizing dsRNA; two antibody-dependent methods (immunoblotting and immunocytochemistry), as well as two antibody-independent methods (differential digestion and acridine orange staining). The benefits and disadvantages of each technique are also discussed.
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Poynter, S.J., DeWitte-Orr, S.J. (2017). Visualizing Virus-Derived dsRNA Using Antibody-Independent and -Dependent Methods. In: Mossman, K. (eds) Innate Antiviral Immunity. Methods in Molecular Biology, vol 1656. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7237-1_5
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DOI: https://doi.org/10.1007/978-1-4939-7237-1_5
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