Abstract
Flow cytometry is a powerful tool for the analysis of apoptosis, the process that directly determines cell fate after the action of different stresses. Here, we describe a flow cytometry method for the assessment of early and late stages of apoptosis in non-fixed cultured cells using SYTO16, DRAQ7, and PO-PRO1 dyes simultaneously. This multicolor flow cytometry procedure requires 45 min for completion and provides a quantitative assessment of cell viability. It can be useful in evaluating the cytotoxic properties of new drugs, and antitumor interventions.
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References
Stokes L, Jiang LH, Alcaraz L et al (2006) Characterization of a selective and potent antagonist of human P2X(7) receptors, AZ11645373. Br J Pharmacol 149:880–887
Wlodkowic D, Skommer J (2007) SYTO probes: markers of apoptotic cell demise. Curr Protoc Cytom 7:7.33
Wlodkowic D, Skommer J, Darzynkiewicz Z (2012) Cytometry of apoptosis. Historical perspective and new advances. Exp Oncol 34:255–262
Akagi J, Kordon M, Zhao H et al (2013) Real-time cell viability assays using a new anthracycline derivative DRAQ7®. Cytometry A 83:227–234
Wlodkowic D, Skommer J, Pelkonen J (2007) Towards an understanding of apoptosis detection by SYTO dyes. Cytometry A 71:61–72
Sparrow RL, Tippett E (2005) Discrimination of live and early apoptotic mononuclear cells by the fluorescent SYTO 16 vital dye. J Immunol Methods 305:173–187
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Kudryavtsev, I., Serebryakova, M., Solovjeva, L., Svetlova, M., Firsanov, D. (2017). Rapid Detection of Apoptosis in Cultured Mammalian Cells. In: Didenko, V. (eds) Fast Detection of DNA Damage. Methods in Molecular Biology, vol 1644. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7187-9_8
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DOI: https://doi.org/10.1007/978-1-4939-7187-9_8
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7185-5
Online ISBN: 978-1-4939-7187-9
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