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Fast Detection of DNA Damage

Methods and Protocols

  • Book
  • © 2017

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1644)

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About this book

This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.

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Keywords

Table of contents (19 protocols)

  1. Express Detection (from 3 min to 1 h)

  2. Rapid Detection (2–3 h)

  3. Accelerated Detection (Time-Saving Versions of Conventional Techniques)

Editors and Affiliations

  • Department of Neurosurgery and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, USA

    Vladimir V. Didenko

Bibliographic Information

  • Book Title: Fast Detection of DNA Damage

  • Book Subtitle: Methods and Protocols

  • Editors: Vladimir V. Didenko

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7187-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7185-5Published: 14 July 2017

  • Softcover ISBN: 978-1-4939-8411-4Published: 11 June 2018

  • eBook ISBN: 978-1-4939-7187-9Published: 13 July 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 216

  • Number of Illustrations: 18 b/w illustrations, 24 illustrations in colour

  • Topics: Human Genetics

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