Abstract
Pollen storage and viability are very important for pollination, breeding, biodiversity, biotechnology, conservation, and other biological and non-biological studies of the date palm. Optimizing procedures and duration of storage are important for effective and long-term date palm pollen storage and viability. Here we describe pollen storage methods, such as room temperature (25–30 °C), refrigeration (4 °C), storage at 4 °C in desiccators, deep freezer (−20 °C), and cryopreservation (−196 °C). Based on pollen viability by staining and in vitro germination methods, cryopreservation is the best method for long-term storage without any significant effect on pollen viability (75–84%); however, the percentage of pollen viability depends on the storage period.
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Acknowledgment
This work was supported by the Higher Education Commission, Pakistan and Plant Tissue Culture Cell, University of Agriculture, Faisalabad 38040, Pakistan.
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Maryam, Jaskani, M.J., Naqvi, S.A. (2017). Storage and Viability Assessment of Date Palm Pollen. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume II. Methods in Molecular Biology, vol 1638. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7159-6_1
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DOI: https://doi.org/10.1007/978-1-4939-7159-6_1
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7159-6
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