Abstract
The emerging field of lipidomics presents the systems biology approach to identify and quantify the full lipid repertoire of cells, tissues, and organisms. The importance of the lipidome is demonstrated by a number of biological studies on dysregulation of lipid metabolism in human diseases such as cancer, diabetes, and neurodegenerative diseases. Exploring changes and regulations in the huge networks of lipids and their metabolic pathways requires a lipidomics methodology: Advanced mass spectrometry that resolves the complexity of the lipidome. Here, we report a comprehensive protocol of quantitative shotgun lipidomics that enables identification and quantification of hundreds of molecular lipid species, covering a wide range of lipid classes, extracted from cultured mammalian cells.
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Acknowledgements
This work was supported by the Scientific Committee of the Danish Cancer Society (KBVU) (K.M) (R124-A7929-15-S2).
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Nielsen, I.Ø., Maeda, K., Bilgin, M. (2017). Global Monitoring of the Mammalian Lipidome by Quantitative Shotgun Lipidomics. In: Bhattacharya, S. (eds) Lipidomics. Methods in Molecular Biology, vol 1609. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6996-8_12
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DOI: https://doi.org/10.1007/978-1-4939-6996-8_12
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