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Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae–Arabidopsis thaliana Interaction

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Auxins and Cytokinins in Plant Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1569))

Abstract

The symptoms of the clubroot disease on Brassica species caused by the obligate biotrophic protist Plasmodiophora brassicae relies, among other factors, on the modulation of plant hormones. Signaling, transport as well as biosynthesis and metabolism are key features how the levels of auxins and cytokinins are controlled. We here describe (a) how to inoculate the model plant Arabidopsis thaliana with P. brassicae, (b) qualitative and quantitative methods to evaluate disease severity in auxin and cytokinin mutants, (c) molecular methods to monitor changes in plant and pathogen transcripts, (d) prerequisites for the establishment of transgenic lines manipulated in an auxin or cytokinin pathway, and (e) methods for β-glucuronidase staining in root galls and sections of infected roots to determine auxin and cytokinin responsive promoter activities.

Susann Auer, Sabine Jülke, and Sabine Marschollek in alphabetical order.

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Authors and Affiliations

  1. Institut für Botanik, Technische Universität Dresden, Zellescher Weg 20b, 01062, Dresden, Germany

    Jutta Ludwig-Müller, Susann Auer, Sabine Jülke & Sabine Marschollek

Authors
  1. Jutta Ludwig-Müller
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  2. Susann Auer
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  3. Sabine Jülke
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  4. Sabine Marschollek
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Corresponding author

Correspondence to Jutta Ludwig-Müller .

Editor information

Editors and Affiliations

  1. Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg Germany, Julius-Maximilians-Universität Würzburg, Würzburg, Germany

    Thomas Dandekar

  2. Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg Germany, Julius-Maximilians-Universität Würzburg, Wurzburg, Germany

    Muhammad Naseem

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Ludwig-Müller, J., Auer, S., Jülke, S., Marschollek, S. (2017). Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae–Arabidopsis thaliana Interaction. In: Dandekar, T., Naseem, M. (eds) Auxins and Cytokinins in Plant Biology. Methods in Molecular Biology, vol 1569. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6831-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6831-2_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6829-9

  • Online ISBN: 978-1-4939-6831-2

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