Abstract
Biosensors are devices that transform a biological interaction into a readout signal, which is evaluable for analytical purposes. The general strength of biosensor approaches is the avoidance of time-consuming and cost-intensive labeling procedures of the analytes. In this chapter, we give insight into a mass-sensitive surface-acoustic wave (SAW) biosensor, which represents an elegant and highly sensitive method to investigate binding events at a molecular level. The principle of SAW technology is based on the piezoelectric properties of the sensors, so as to binding events and their accompanied mass increase at the sensor surface are detectable by a change in the oscillation of the surface acoustic wave. In combination with model membranes, transferred to the sensor surface, the analytical value of SAW biosensors has strongly been increased and extended to different topics of biomedical investigations, including antibiotic research. The interaction with the bacterial membrane or certain target structures therein is the essential mode of action for various antibacterial compounds. Beside targeted interaction, an unspecific membrane binding or membrane insertion of drugs can contribute to the antibacterial activity by changing the lateral order of membrane constituents or by interfering with the membrane barrier function. Those pleiotropic effects are hardly to illustrate in the bacterial systems and need a detailed view at the in vitro level. Here, we illustrate the usefulness of a SAW biosensor in combination with model membranes to investigate the mode of membrane interaction of antibiotic active peptides. Using two different peptides we exemplary describe the interaction analysis in a two-step gain of information: (1) a binding intensity or affinity by analyzing the phase changes of oscillation, and (2) mode of membrane interaction, i.e., surface binding or internalization of the peptide by following the amplitude of oscillation.
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References
Nguyen HH, Park J, Kang S, Kim M (2015) Surface plasmon resonance: a versatile technique for biosensor applications. Sensors 15:10481–10510
Gronewold TMA (2007) Surface acoustic wave sensors in the bioanalytical field: recent trends and challenges. Anal Chim Acta 603:119–128
Gronewold TMA, Schlecht U, Quandt E (2007) Analysis of proteolytic degradation of a crude protein mixture using a surface acoustic wave sensor. Biosens Bioelectron 22:2360–2365
Länge K, Rapp BE, Rapp M (2008) Surface acoustic wave biosensors: a review. Anal Bioanal Chem 391:1509–1519
Schlesinger M, Simonis D, Schmitz P, Fritzsche J, Bendas G (2009) Binding between heparin and the integrin VLA-4. Thromb Haemost 102:816–822
Baca JT, Severns V, Lovato D, Branch DW, Larson RS (2015) Rapid detection of Ebola virus with a reagent-free, point-of-care biosensor. Sensors 15:8605–8614
Slamnoiu S, Vlad C, Stumbaum M, Moise A, Lindner K, Engel N et al (2014) Identification and affinity-quantification of ß-amyloid and α-synuclein polypeptides using on-line SAW-biosensor-mass spectrometry. J Am Soc Mass Spectrom 8:1472–1481
Engelman DM (2005) Membranes are more mosaic than fluid. Nature 438:578–580
Lee TH, Hall KN, Aguilar MI (2016) Antimicrobial peptide structure and mechanism of action: a focus on the role of membrane structure. Curr Top Med Chem 16:25–39
Schneider T, Sahl HG (2010) Lipid II and other bactoprenol-bound cell wall precursors as drug targets. Curr Opin Investig Drugs 11:157–164
Reder-Christ K, Schmitz P, Bota M, Gerber U, Falkenstein-Paul H, Fuss C et al (2013) A dry membrane protection technique to allow surface acoustic wave biosensor measurements of biological model membrane approaches. Sensors 13:12392–12405
Gerber U, Hoß SG, Shteingauz A, Jüngel E, Jakubzig B, Ilan N et al (2015) Latent heparanase facilitates VLA-4-mediated melanoma cell binding and emerges as a relevant target of heparin in the interference with metastatic progression. Semin Thromb Hemost 41:244–254
Girard-Egrot AP, Blum LJ (2006) Langmuir-Blodgett technique for synthesis of biomimetic lipid membranes. In: Martin D (ed) Nanotechnology of biomimetic membranes, 1st edn. Springer Science+Business Media LLC, New York, pp 23–74
Acknowledgments
The authors would like to thank Hildegard Falkenstein-Paul for excellent technical competences in performing the SAW measurements and providing the data presented here.
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Hoß, S.G., Bendas, G. (2017). Mass-Sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes. In: Sass, P. (eds) Antibiotics. Methods in Molecular Biology, vol 1520. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6634-9_9
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DOI: https://doi.org/10.1007/978-1-4939-6634-9_9
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Publisher Name: Humana Press, New York, NY
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