Abstract
Microsatellites are polymorphic DNA loci comprising repeated sequence motifs of two to five base pairs which are dispersed throughout the genome. Genotyping of microsatellites is a widely accepted tool for diagnostic and research purposes such as forensic investigations and parentage testing, but also in clinics (e.g. monitoring of bone marrow transplantation), as well as for the agriculture and food industries. The co-amplification of several short tandem repeat (STR) systems in a multiplex reaction with simultaneous detection helps to obtain more information from a DNA sample where its availability may be limited. Here, we introduce and describe this commonly used genotyping technique, providing an overview on available resources on STRs, multiplex design, and analysis.
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Acknowledgements
We would like to cordially thank Peter Kovacs, head of the research group Genetics of Obesity and Diabetes, and our colleagues for their everlasting scientific and personal support. We thank Mohammed Hankir for proofreading of this manuscript.
Funding
Jacqueline Krüger is funded by a Collaborative Research Center (B03, CRC1052) granted by the German Research Foundation (DFG). Dorit Schleinitz is funded by the Boehringer Ingelheim Foundation.
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Krüger, J., Schleinitz, D. (2017). Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems. In: White, S., Cantsilieris, S. (eds) Genotyping. Methods in Molecular Biology, vol 1492. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6442-0_1
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DOI: https://doi.org/10.1007/978-1-4939-6442-0_1
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