Abstract
Having the ability to rapidly, accurately, and robustly measure Plasmodium falciparum merozoite invasion is a critical component in effective assessment of a blood stage vaccine’s mechanism of action. Being able to measure invasion of erythrocytes accurately, objectively and in a high throughput fashion is of critical importance. Here, we describe a simple and robust flow cytometry method that allows for the measurement of the key invasion parameters of parasite multiplication rate and erythrocyte selectivity—both important determinants of disease severity—from the schizont to the ring stage of the parasite’s life-cycle, thus separating invasion from growth of the parasite. Importantly, this method is able to accurately detect low levels of parasitemia and heterogeneity within the population that can be missed by enzymatic methods. Lastly, this method has been successfully adapted and employed in field based research settings for parasitemia measurements in vivo, ex vivo, and in vitro and to measure invasion inhibition by antibodies and the use of alternative pathways for invasion.
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The authors would like to acknowledge Dr. Jeffrey Dvorin for critical reading of the manuscript.
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Bei, A.K., Duraisingh, M.T. (2015). Measuring Plasmodium falciparum Erythrocyte Invasion Phenotypes Using Flow Cytometry. In: Vaughan, A. (eds) Malaria Vaccines. Methods in Molecular Biology, vol 1325. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2815-6_14
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DOI: https://doi.org/10.1007/978-1-4939-2815-6_14
Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-2815-6
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