Abstract
This simple, versatile, reliable, reproducible, multipurpose, and inexpensive technique is based on the adhesion of different antigens to a single nitrocellulose strip using, as template, an acrylic device containing 28 parallel channels. The inclusion of channels containing normal human serum improves the quality control of this assay. Antigen-sensitized nitrocellulose strips are cut perpendicularly to the antigen-rows, exposed to immune sera followed by the appropriate conjugate. Positive signals are recorded using chemiluminescent or precipitable colorimetric substrates. This assay allows the simultaneous qualitative demonstration of antigenicity and immunogenicity of antigens obtained as synthetic peptides, recombinant molecules, or crude preparations, with high sensitivity and specificity. Its major value is based on the rapid and simultaneous comparative evaluation of various antigenic preparations allowing the diagnosis of a variety of infectious, allergic, and autoimmune diseases. It can in general be used to detect any type of antibody or circulating antigen. Some improvements and variants of the original technique are included.
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Acknowledgment
This work has been partially supported by grants N° G-2005000387, 201100413, 2007001425, F-2005000122 and 20122001311 from FONACIT-Venezuela. We also thank the Consejo de Desarrollo Científico y Humanístico de la UCV for financing our participation in the “XVIII International Congress of Tropical Medicine and Malaria” in Brazil, with the conference “The simultaneous multidiagnosis of infectious agents as a routine laboratory screening test for the near future” (ICC09-0142-2012). We thank Dr. Peter Taylor, for help with the manuscript.
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Noya, O. et al. (2015). Improvements and Variants of the Multiple Antigen Blot Assay-MABA: An Immunoenzymatic Technique for Simultaneous Antigen and Antibody Screening. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_32
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DOI: https://doi.org/10.1007/978-1-4939-2694-7_32
Publisher Name: Humana Press, New York, NY
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