Abstract
Antigen detection is a well-known tool in the scientific world that is used by clinicians and researchers to detect specific antigens in diagnosing diseases or for other medical/environmental discoveries. Antigen detection is introduced in various forms over the past decades. These techniques are often evaluated by their sensitivity, accuracy, and ease of use. One technique that has provided many advantages over typical immunochemical staining is the use of chemiluminescence. This technique has been used in various scientific fields, anywhere from clinical diagnosis to environmental research. The emission of visible radiation by compounds once exposed to sunlight has been known for centuries and currently is the main principle for chemiluminescence. Here, we introduce three different chemiluminescence techniques that are widely used in immunodetection of antigens: (a) whole membrane chemiluminescence detection, (b) strip membrane chemiluminescence detection, and (c) new-line blotting chemiluminescence.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
García-Campaña AM, Baeyens WRG (eds) (2001) Chemiluminescence in analytical chemistry. Marcel Dekker, Inc., New York, NY
Dorri Y, Kurien BT, Scofield RH (2009) A simpler and faster version of two-dimensional gel electrophoresis using vertical, mini SDS-PAGE apparatus. Iran J Chem Chem Eng 28:51–56
Tong X, Sheng P, Yan Z, Tran TT, Wang X, Cai J, Cai Q (2014) Core/shellthick CdTe/CdS quantum dots functionalized TiO2 nanotube: a novel electrochemiluminescence platform for label-free immunosensor to detect tris-(2,3-dibromopropyl) isocyanurate in environment. Sens Actuators B 198:41–48
Chen F, Mao S, Zeng H, Xue S, Yang J, Nakajima H et al (2013) Inkjet nanoinjection for high-throughput chemiluminescence immunoassay on multicapillary glass plate. Anal Chem 85:7413–7418
Xin TB, Liang SX, Wang X, Li H, Lin JM (2008) Determination of estradiol in human serum using magnetic particles-based chemiluminescence immunoassay. Anal Chim Acta 627:277–284
Heider H, Ziaja B, Priemer C, Lundkvist A, Neyts J, Krüger DH et al (2001) A chemiluminescence detection method of hantaviral antigens in neutralisation assays and inhibitor studies. J Virol Methods 96:17–23
Deluca MA, Mcelroy WD (1981) Bioluminescence and chemiluminescence. Basic chemistry and analytical applications. Academic, New York, NY
Tsukagoshi K, Tsuge K, Nakajima R (2007) Development of an immune microanalysis system by use of peroxyoxalate chemiluminescence detection. Anal Sci 23:739–741
Crisp JS, Dunn JM (1994) Detection of protein blots using chemiluminescent systems. In: Walker JM (ed) Methods in biology. Humana Press Inc., Totowa, NJ, pp 233–237
Kurien BT, Scofield RH (1997) Multiple immunoblots after non-electrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple antigens. J Immunol Methods 205:91–94
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Dorri, Y. (2015). Immunoblotting of Antigens: Whole, Strip, and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique. In: Kurien, B., Scofield, R. (eds) Detection of Blotted Proteins. Methods in Molecular Biology, vol 1314. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2718-0_10
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2718-0_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2717-3
Online ISBN: 978-1-4939-2718-0
eBook Packages: Springer Protocols