Abstract
Creating transgenic plants is invaluable for the genetic analysis of sugar beet and will be increasingly important as sugar beet genomic technologies progress. A protocol for Agrobacterium-mediated transformation of sugar beet is described in this chapter. Our protocol is optimized for a sugar beet genotype that performs exceptionally well in tissue culture, including the steps of dedifferentiation, callus proliferation, and regeneration. Because of the infrequent occurrence of such a genotype in sugar beet populations, our protocol includes an in vitro propagation method for germplasm preservation. The starting materials for transgenic experiments are aseptic shoots grown from surface-sterilized seed balls. Callus is induced from leaf explants and subsequently infected with Agrobacterium. Plantlets are regenerated from transgenic callus and vernalized for flowering, if necessary. The efficiency of transformation was quite high; in our laboratory, the culture of only ten leaf explants, on average, generated one transgenic plant.
The original version of this chapter was revised: See the “Erratum” chapter near the end of the book for a complete listing of the revisions
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-1695-5_29
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Acknowledgments
The authors wish to thank Ms. Chisato Osawa for her technical assistance. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology, Japan; Grants-in-Aid for science and technology research program for agriculture, forestry, fisheries and food industry, Japan; and Grants-in-Aid for Scientific Research from the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN).
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Kagami, H. et al. (2015). Sugar Beet (Beta vulgaris L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_27
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DOI: https://doi.org/10.1007/978-1-4939-1695-5_27
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