Abstract
Until now, the application of genetic transformation techniques in peach has been limited by the difficulties in developing efficient regeneration and transformation protocols. Here we describe an efficient regeneration protocol for the commercial micropropagation of GF677 rootstock (Prunus persica × Prunus amygdalus). The method is based on the production, via organogenesis, of meristematic bulk tissues characterized by a high competence for shoot regeneration.
This protocol has also been used to obtain GF677 plants genetically engineered with an empty hairpin cassette (hereafter indicated as hp-pBin19), through Agrobacterium tumefaciens-mediated transformation. After 7–8 months of selection on media containing kanamycin, we obtained two genetically modified GF677 lines. PCR and Southern blot analyses were performed to confirm the genetic status.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Meng X, Zhou W (1981) Induction of embryoid ad production of plantlets in vitro from endosperm of peach. Acta Agric Univ Peking 7:95–98
Hammerschlag FA, Bauchan G, Scorza R (1985) Regeneration of peach plants from callus derived from immature embryos. Theor Appl Genet 70:248–251
Mante S, Scorza R, Cordts JM (1989) Plant regeneration from cotyledons of Prunus persica. Prunus domestica and Prunus cerasus. Plant Cell Tiss Org Cult 19:1–11
Scorza R, Morgens PH, Cordts JM, Mante S, Callahan AM (1990) Agrobacterium-mediated transformation of peach Prunus persica L. Batsch leaf segments, immature embryos and long term embryogenic callus. In Vitro Cell Dev Biol 26:829–834
Bhansali RR, Driver JA, Durzan DJ (1990) Rapid multiplication of adventitious somatic embryos in peach and nectarine by secondary embryogenesis. Plant Cell Rep 9:280–284
Pooler MR, Scorza R (1995) Regeneration of peach Prunus persica L. Batsch rootstock cultivars from cotyledons of mature stored seed. HortSci 30:355–356
Zhou HC, Li M, Zhao X, Fan XC, Guo AG (2010) Plant regeneration from in vitro leaves of the peach rootstock ‘Nemaguard’ (Prunus persica x P. davidiana). Plant Cell Tiss Org Cult 101:79–87
Pérez-Jiménez M, Carrillo-Navarro A, Cos-Terrer J (2012) Regeneration of peach (Prunus persica L. Batsch) cultivars and Prunus persica x Prunus dulcis rootstocks via organogenesis. Plant Cell Tissue Organ Cult 108:55–62
Mezzetti B, Pandolfini T, Navacchi O, Landi L (2002) Genetic transformation of Vitis vinifera via organogenesis. BMC Biotechnol 2:18
Smigocki AC, Freddi A, Hammerschlag A (1991) Regeneration of plants from peach embryo cells infected with a shooty mutant strain of Agrobacterium. J Am Soc HorticSci 116:1092–1097
Pérez-Clemente R, Pérez-Sanjuán A, García-Férriz L, Beltrán J-P, Cañas LA (2004) Transgenic peach plants (Prunus persica L.) produced by genetic transformation of embryo sections using the green fluorescent protein (GFP) as an in vivo marker. Mol. Breed 14:419–427
Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12:8711–8721
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
Compton ME, Gray DJ (1993) Shoot organogenesis and plant regeneration from cotyledons of diploid, triploid, and tetraploid watermelon. J Am Soc HortSci 118:151–157
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Sabbadini, S., Pandolfini, T., Girolomini, L., Molesini, B., Navacchi, O. (2015). Peach (Prunus persica L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1224. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1658-0_17
Download citation
DOI: https://doi.org/10.1007/978-1-4939-1658-0_17
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4939-1657-3
Online ISBN: 978-1-4939-1658-0
eBook Packages: Springer Protocols