Abstract
In the last 20 years, the development of murine models of allergic asthma has provided researchers with a means to explore the mechanisms of this T-helper type 2 (Th2)-driven inflammatory disease. While systemic sensitization and airway challenge with ovalbumin has been the most widely used model, recent emphasis has been placed on the development of models using more naturally occurring antigens. However, the diversity of models currently available makes it hard for investigators new to this field to choose to use the most effective and appropriate model to test their hypothesis. Here we describe three different mouse models of allergic asthma, including the classical ovalbumin model, a modified ovalbumin model that has been shown to be mast-cell dependent, as well as a house dust mite antigen-induced model. We also discuss briefly their characterization and differences, in the aim to facilitate the choice of the appropriate model when working on this intricate Th2 inflammatory disease.
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References
Barnes PJ (2008) Immunology of asthma and chronic obstructive pulmonary disease. Nat Rev Immunol 8:183–192. doi:10.1038/nri2254
Lee JJ et al (2004) Defining a link with asthma in mice congenitally deficient in eosinophils. Science 305:1773–1776. doi:10.1126/science.1099472
Fulkerson PC et al (2006) A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation. Proc Natl Acad Sci U S A 103:16418–16423. doi:10.1073/pnas.0607863103
Walsh ER et al (2008) Strain-specific requirement for eosinophils in the recruitment of T cells to the lung during the development of allergic asthma. J Exp Med 205:1285–1292. doi:10.1084/jem.20071836
Fattouh R et al (2011) Eosinophils are dispensable for allergic remodeling and immunity in a model of house dust mite-induced airway disease. Am J Respir Crit Care Med 183:179–188. doi:10.1164/rccm.200905-0736OC
Corry DB et al (1998) Requirements for allergen-induced airway hyperreactivity in T and B cell-deficient mice. Mol Med 4:344–355
Becker M et al (2011) Genetic variation determines mast cell functions in experimental asthma. J Immunol 186:7225–7231. doi:10.4049/jimmunol.1100676
Nakae S et al (2007) Mast cell-derived TNF contributes to airway hyperreactivity, inflammation, and TH2 cytokine production in an asthma model in mice. J Allergy Clin Immunol 120:48–55. doi:10.1016/j.jaci.2007.02.046
Desquand S, Lefort J, Liu FT, Mencia-Huerta JM, Vargaftig BB (1989) Antigen-induced bronchopulmonary alterations in the guinea pig: a new model of passive sensitization mediated by mouse IgE antibodies. Int Arch Allergy Appl Immunol 89:71–77
Sertl K, Kowalski ML, Slater J, Kaliner MA (1988) Passive sensitization and antigen challenge increase vascular permeability in rat airways. Am Rev Respir Dis 138:1295–1299
Van Oosterhout AJ et al (1993) Effect of anti-IL-5 and IL-5 on airway hyperreactivity and eosinophils in guinea pigs. Am Rev Respir Dis 147:548–552
Lopez E et al (2011) Gene expression profiling in lungs of chronic asthmatic mice treated with galectin-3: downregulation of inflammatory and regulatory genes. Mediators Inflamm 2011:823279. doi:10.1155/2011/823279
Yu M et al (2011) Identification of an IFN-gamma/mast cell axis in a mouse model of chronic asthma. J Clin Invest 121:3133–3143. doi:10.1172/jci43598
Kung TT et al (1995) Mast cells modulate allergic pulmonary eosinophilia in mice. Am J Respir Cell Mol Biol 12:404–409
Takeda K et al (1997) Development of eosinophilic airway inflammation and airway hyperresponsiveness in mast cell-deficient mice. J Exp Med 186:449–454
Williams CM, Galli SJ (2000) Mast cells can amplify airway reactivity and features of chronic inflammation in an asthma model in mice. J Exp Med 192:455–462
Cho JY et al (2010) Chronic OVA allergen challenged Siglec-F deficient mice have increased mucus, remodeling, and epithelial Siglec-F ligands which are up-regulated by IL-4 and IL-13. Respir Res 11:154. doi:10.1186/1465-9921-11-154
Cho JY et al (2004) Inhibition of airway remodeling in IL-5-deficient mice. J Clin Invest 113:551–560. doi:10.1172/jci19133
O’Brien R, Ooi MA, Clarke AH, Thomas WR (1996) Immunologic responses following respiratory sensitization to house dust mite allergens in mice. Immunol Cell Biol 74:174–179. doi:10.1038/icb.1996.24
Cates EC et al (2004) Intranasal exposure of mice to house dust mite elicits allergic airway inflammation via a GM-CSF-mediated mechanism. J Immunol 173:6384–6392
Jacquet A (2011) The role of innate immunity activation in house dust mite allergy. Trends Mol Med 17:604–611. doi:10.1016/j.molmed.2011.05.014
Thomas WR, Smith WA, Hales BJ, Mills KL, O’Brien RM (2002) Characterization and immunobiology of house dust mite allergens. Int Arch Allergy Immunol 129:1–18
Phipps S et al (2009) Toll/IL-1 signaling is critical for house dust mite-specific helper T cell type 2 and type 17 [corrected] responses. Am J Respir Crit Care Med 179:883–893. doi:10.1164/rccm.200806-974OC
Melgert BN et al (2010) Macrophages: regulators of sex differences in asthma? Am J Respir Cell Mol Biol 42:595–603. doi:10.1165/rcmb.2009-0016OC
Melgert BN et al (2005) Female mice are more susceptible to the development of allergic airway inflammation than male mice. Clin Exp Allergy 35:1496–1503. doi:10.1111/j.1365-2222.2005.02362.x
van Rijt LS et al (2004) A rapid flow cytometric method for determining the cellular composition of bronchoalveolar lavage fluid cells in mouse models of asthma. J Immunol Methods 288:111–121. doi:10.1016/j.jim.2004.03.004
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Gold, M., Marsolais, D., Blanchet, MR. (2015). Mouse Models of Allergic Asthma. In: Hughes, M., McNagny, K. (eds) Mast Cells. Methods in Molecular Biology, vol 1220. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1568-2_31
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DOI: https://doi.org/10.1007/978-1-4939-1568-2_31
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