Abstract
Mouse models of allergic asthma have been utilized to establish the role of T helper type 2 (Th2) cells in driving lung inflammation, airway hyperresponsiveness, and obstruction. Here, we present the allergic asthma models, in which mice are hypersensitized to ovalbumin (OVA) and house dust mite (HDM). These models mimic the major characteristics of human asthma including the eosinophilic inflammation and hyperactivity of the airway, overproduction of Th2 cytokines in the lung, and elevated total and allergen-specific immunoglobulin E (IgE) in serum.
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References
Lambrecht BN, Hammad H (2015) The immunology of asthma. Nat Immunol 16:45–56
Akdis CA (2006) Allergy and hypersensitivity: mechanisms of allergic disease. Curr Opin Immunol 18:718–726
Broide DH (2001) Molecular and cellular mechanisms of allergic disease. J Allergy Clin Immunol 108:S65–S71
Busse WW, Lemanske RF Jr (2001) Asthma. N Engl J Med 344:350–362
Nakajima H, Takatsu K (2007) Role of cytokines in allergic airway inflammation. Int Arch Allergy Immunol 142:265–273
Wakashin H, Hirose K, Maezawa Y et al (2008) IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice. Am J Respir Crit Care Med 178:1023–1032
Zosky GR, Sly PD (2007) Animal models of asthma. Clin Exp Allergy 37:973–988
Chapman DG, Tully JE, Nolin JD et al (2014) Animal models of allergic airways disease: where are we and where to next? J Cell Biochem 115:2055–2064
Cho S, Wu CJ, Yasuda T et al (2016) miR-23∼27∼24 clusters control effector T cell differentiation and function. J Exp Med 213:235–249
Miller M, Beppu A, Rosenthal P et al (2015) Fstl1 promotes asthmatic airway remodeling by inducing oncostatin M. J Immunol 195:3546–3556
O’Byrne PM, Inman MD (2003) Airway hyperresponsiveness. Chest 123:411S–416S
McBrien CN, Menzies-Gow A (2017) The biology of eosinophils and their role in asthma. Front Med 4:93. https://doi.org/10.3389/fmed.2017.00093
Fischer AH, Jacobson KA, Rose J et al (2008) Hematoxylin and eosin staining of tissue and cell sections. CSH Protoc 2008:pdb.prot4986. https://doi.org/10.1101/pdb.prot4986
Dabbagh K, Takeyama K, Lee HM et al (1999) IL-4 induces mucin gene expression and goblet cell metaplasia in vitro and in vivo. J Immunol 162:6233–6237
Lloyd CM, Hessel EM (2010) Functions of T cells in asthma: more than just T(H)2 cells. Nat Rev Immunol 10:838–848
Dullaers M, De Bruyne R, Ramadani F et al (2012) The who, where, and when of IgE in allergic airway disease. J Allergy Clin Immunol 2012(129):635–645
Wu CJ, Yang CY, Chen YH et al (2013) The DNA methylation inhibitor 5-azacytidine increases regulatory T cells and alleviates airway inflammation in ovalbumin-sensitized mice. Int Arch Allergy Immunol 160:356–364
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This work was supported by NIH grant AI108651.
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Chen, MC., Tai, J.W., Wu, CJ. (2021). Induction of Airway Hypersensitivity to Ovalbumin and Dust Mite Allergens as Mouse Models of Allergic Asthma. In: Nagamoto-Combs, K. (eds) Animal Models of Allergic Disease. Methods in Molecular Biology, vol 2223. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1001-5_8
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DOI: https://doi.org/10.1007/978-1-0716-1001-5_8
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