Abstract
Double-stranded RNA (dsRNA) is the replicate intermediate of all RNA viruses, and is also recognized by their host cells as a virus-invading molecule signal. Analysis of the localization and dynamic of virus-induced dsRNA can reveal crucial information concerning the molecular mechanism of virus replication and host responses to viral infection. In this chapter, we provide an easy and efficient protocol called dsRNA binding-dependent fluorescence complementation (dRBFC) assay for labeling the dsRNAs in living plant cells using two different plant RNA viruses, namely potato virus X and turnip mosaic virus. Moreover, both YFP- and mRFP-based dRBFC plasmids are available for the flexibility of experiment design.
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Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (32022071) to XC.
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Zhang, Y., Fan, X., Cheng, X. (2024). Analysis of Virus-Induced Double-Stranded RNA in Living Plant Cells by the dRBFC Assay. In: Cheng, X., Wu, G. (eds) Double-Stranded RNA. Methods in Molecular Biology, vol 2771. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3702-9_5
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DOI: https://doi.org/10.1007/978-1-0716-3702-9_5
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3701-2
Online ISBN: 978-1-0716-3702-9
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