Abstract
Due to the highly conserved genetics across the central nervous system, the easily probed visual system can act as an endophenotype for assessing neurological function. Here, we describe a psychophysics approach to assess visually driven swimming behavior in the high-throughput zebrafish genetic model system. We use the optomotor response test together with general locomotion behavior to assess neural processing while excluding motor defects related to muscle function.
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References
Curpan AS, Balmus IM, Dobrin RP et al (2022) A mini-review regarding the modalities to study neurodevelopmental disorders-like impairments in zebrafish-focussing on neurobehavioural and psychological responses. Brain Sci 12(9). https://doi.org/10.3390/brainsci12091147
Bilotta J, Saszik S (2001) The zebrafish as a model visual system. Int J Dev Neurosci 19(7):621–629. https://doi.org/10.1016/s0736-5748(01)00050-8
Chhetri J, Jacobson G, Gueven N (2014) Zebrafish--on the move towards ophthalmological research. Eye (Lond) 28(4):367–380. https://doi.org/10.1038/eye.2014.19
Gestri G, Link BA, Neuhauss SC (2012) The visual system of zebrafish and its use to model human ocular diseases. Dev Neurobiol 72(3):302–327. https://doi.org/10.1002/dneu.20919
Easter SS Jr, Malicki JJ (2002) The zebrafish eye: developmental and genetic analysis. Results Probl Cell Differ 40:346–370. https://doi.org/10.1007/978-3-540-46041-1_17
Xie J, Jusuf PR, Bui BV et al (2019) Experience-dependent development of visual sensitivity in larval zebrafish. Sci Rep 9(1):18931. https://doi.org/10.1038/s41598-019-54958-6
Orger MB, Gahtan E, Muto A et al (2004) Behavioral screening assays in zebrafish. Methods Cell Biol 77:53–68. https://doi.org/10.1016/s0091-679x(04)77003-x
Sztal TE, Ruparelia AA, Williams C et al (2016) Using touch-evoked response and locomotion assays to assess muscle performance and function in zebrafish. J Vis Exp 116. https://doi.org/10.3791/54431
Neuhauss SC (2003) Behavioral genetic approaches to visual system development and function in zebrafish. J Neurobiol 54(1):148–160. https://doi.org/10.1002/neu.10165
Neuhauss SC, Biehlmaier O, Seeliger MW et al (1999) Genetic disorders of vision revealed by a behavioral screen of 400 essential loci in zebrafish. J Neurosci 19(19):8603–8615. https://doi.org/10.1523/JNEUROSCI.19-19-08603.1999
Mario K, David B, Denis P et al (2007) What’s new in Psychtoolbox-3. Perception 36(14):1–1
Bizrah M, Dakin SC, Guo L et al (2014) A semi-automated technique for labeling and counting of apoptosing retinal cells. BMC Bioinf 15(1):1–12
Lu Z-L, Dosher B (2013) Visual psychophysics: from laboratory to theory. MIT Press
D'AGOSTINO R, Pearson ES (1973) Tests for departure from normality. Empirical results for the distributions of b 2 and√ b. Biometrika 60(3):613–622
Dunn TW, Fitzgerald JE (2020) Correcting for physical distortions in visual stimuli improves reproducibility in zebrafish neuroscience. eLife 9:e53684. https://doi.org/10.7554/eLife.53684
Creton R (2009) Automated analysis of behavior in zebrafish larvae. Behav Brain Res 203(1):127–136. https://doi.org/10.1016/j.bbr.2009.04.030
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Xie, J., Goodbourn, P., Sztal, T., Jusuf, P.R. (2024). Neural Endophenotype Assessment in Zebrafish Larvae Using Optomotor and ZebraBox Locomotion Assessment. In: Dworkin, S. (eds) Neurobiology. Methods in Molecular Biology, vol 2746. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3585-8_17
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DOI: https://doi.org/10.1007/978-1-0716-3585-8_17
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