Abstract
Fourier transform mid-infrared spectroscopy (FTIR) is a powerful tool for compositional analysis of plant cell walls. The infrared spectrum generates a fingerprint of a sample with absorption peaks corresponding to the frequency of vibrations between the bonds of the atoms making up the material. Here we describe a method focused on the use of FTIR in combination with principal component analysis (PCA) to characterize the composition of the plant cell wall. The FTIR method described here facilitates high-throughput identification of the major compositional differences across a large set of samples in a low-cost and non-destructive manner.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Ribeiro GO, Gruninger RJ, Badhan A, McAllister TA (2016) Mining the rumen for fibrolytic feed enzymes. Anim Front 6:20–26
Badhan A, McAllister TA (2015) Designer plants for biofuel: a review. Curr Metab 4(1):49–55
Badhan AK, Chadha BS, Kaur J, Sonia KG, Saini HS, Bhat MK (2007) Role of transglycosylation products in the expression of multiple xylanases in Myceliophthora sp. IMI 387099. Curr Microbiol 54:405–409
Baker MJ, Trevisan J, Bassan P, Bhargava R, Butler HJ, Dorling KM, Fielden PR, Fogarty SW, Fullwood NJ, Heys KA, Hughes C, Lasch P, Martin-Hirsch PL, Obinaju B, Sockalingum GD, Sulé-Suso J, Strong RJ, Walsh MJ, Wood BR, Gardner P, Martin FL (2014) Using Fourier transform IR spectroscopy to analyze biological materials. Nat Protocols 9:1771–1791
Allison GG, Thain SC, Morris P, Morris C, Hawkins S, Hauck B, Barraclough T, Yates N, Shield I, Bridgwater AV, Donnison IS (2009) Quantification of hydroxycinnamic acids and lignin in perennial forage and energy grasses by Fourier-transform infrared spectroscopy and partial least squares regression. Bioresour Technol 100:1252–1261
Carpita NC, McCann MC (2015) Characterizing visible and invisible cell wall mutant phenotypes. J Exp Bot 66:4145–4163
Chen L, Carpita NC, Reiter WD, Wilson RH, Jeffries C, McCann MC (1998) A rapid method to screen for cell-wall mutants using discriminant analysis of Fourier transform infrared spectra. Plant J 16:385–392
Jitraporn V, David PG, Bayden RW, Philip H, Karina K, David H, Mark JH, Mark KT (2019) Synchrotron macro-ATR-FTIR micro spectroscopy for high-resolution chemical mapping of single cell. Analyst 144:3226
Holman HY, Bechtel HA, Hao Z, Martin MC (2010) Synchrotron IR Spectromicroscopy: chemistry of living cells. Anal Chem 82(21):8757–8765
Acknowledgment
The authors wish to acknowledge RDAR, Alberta Milk, and Alberta Agriculture and Forestry (proposal no: 2022F105R and 2021F041R) for their generous support of their research program.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Badhan, A., Wang, Y., McAllister, T.A. (2023). Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transform Mid-Infrared Spectroscopy. In: Abbott, D.W., Zandberg, W.F. (eds) Carbohydrate-Protein Interactions. Methods in Molecular Biology, vol 2657. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3151-5_15
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3151-5_15
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3150-8
Online ISBN: 978-1-0716-3151-5
eBook Packages: Springer Protocols