Abstract
Growing adipose-derived stem cells (ADSC) in serum-free conditions is important as it represents a way of expanding multipotent cells in a clinical grade medium. Most cultured ADSC are expanded and tested in serum-containing media, which can pose significant health risks if these cells were used in clinical applications. Moreover, cells grown in serum-free conditions behave very different than those cultured in serum-containing media. Here, we present a technique to culture adipose-derived stem cells in serum-free conditions. The methods described in this chapter were optimized for ovine ADSC. The appropriate optimization should be done for other cell lines.
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Acknowledgments
The financial support provided by grants from the New Zealand Lottery Board (Lottery Health Research Grant) and the Otago Medical Research Foundation (Jack Thompson Arthritis Grant), and a University of Otago Doctoral Scholarship (awarded to D.G.Z.) are gratefully acknowledged. We also thank the Molecular Biosciences Laboratory (Faculty of Dentistry) personnel.
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Zanicotti, D.G., Milne, T.J., Coates, D.E. (2023). Culturing Adipose-Derived Stem Cells Under Serum-Free Conditions. In: Seymour, G.J., Cullinan, M.P., Heng, N.C., Cooper, P.R. (eds) Oral Biology. Methods in Molecular Biology, vol 2588. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2780-8_23
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DOI: https://doi.org/10.1007/978-1-0716-2780-8_23
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