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Methods to Study West Nile Virus Infection and the Virus-Induced Inflammation in the Brain in a Murine Model

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West Nile Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2585))

Abstract

West Nile virus (WNV), a mosquito-borne neurotropic flavivirus, has become the leading cause of vector-borne viral encephalitis in the United States for the past decades. The murine model of WNV infection is an effective in vivo experimental model to investigate WNV neuropathogenesis in humans. Here, we describe several laboratory protocols to study WNV infection and the virus-induced inflammation in the brain in both in vitro and in vivo murine models.

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Acknowledgments

This work was supported in part by NIH grants R01 AI127744 (T.W.) and R21 AI140569 (T.W.).

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Authors and Affiliations

  1. School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China

    Huanle Luo

  2. Key Laboratory of Tropical Disease Control, Sun Yat-sen University, Ministry of Education, Guangzhou, China

    Huanle Luo

  3. Department of Microbiology & Immunology, University of Texas Medical Branch, Galveston, TX, USA

    Tian Wang

  4. Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA

    Tian Wang

  5. Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA

    Tian Wang

Authors
  1. Huanle Luo
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  2. Tian Wang
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Corresponding author

Correspondence to Tian Wang .

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Editors and Affiliations

  1. Department of Cell and Molecular Biology, Center for Molecular and Cellular Biosciences The University of Southern Mississippi, Hattiesburg, MS, USA

    Fengwei Bai

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Luo, H., Wang, T. (2023). Methods to Study West Nile Virus Infection and the Virus-Induced Inflammation in the Brain in a Murine Model. In: Bai, F. (eds) West Nile Virus. Methods in Molecular Biology, vol 2585. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2760-0_6

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  • DOI: https://doi.org/10.1007/978-1-0716-2760-0_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2759-4

  • Online ISBN: 978-1-0716-2760-0

  • eBook Packages: Springer Protocols

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