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Electrophysiology of Endocannabinoid Signaling

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Endocannabinoid Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2576))

Abstract

Electrophysiological technique is an efficient tool for investigating the synaptic regulatory effects mediated by the endocannabinoid system. Stimulation of presynaptic type 1 cannabinoid receptor (CB1) is the principal mode by which endocannabinoids suppress transmitter release in the central nervous system, but a non-retrograde manner of functioning and other receptors have also been described. Endocannabinoids are key modulators of both short- and long-term plasticity. Here, we discuss ex vivo electrophysiological approaches to examine synaptic signaling induced by cannabinoid and endocannabinoid molecules in the mammalian brain.

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Acknowledgments

The authors were supported by Italian Ministry of Health (GR-2016-02361163 to AM; RF-2018-12366144 to DC), Ricerca corrente of IRCCS San Raffaele Roma, Ricerca corrente of IRCCS Neuromed, Italian Ministry of University and Research (PRIN 2017- cod. 2017K55HLC to DC), and FISM-Fondazione Italiana Sclerosi Multipla (cod. 2019/S/1).

Author information

Authors and Affiliations

  1. Synaptic Immunopathology Lab, IRCCS San Raffaele Roma, Rome, Italy

    Alessandra Musella

  2. Department of Human Sciences and Quality of Life Promotion University of Rome San Raffaele, Rome, Italy

    Alessandra Musella

  3. Department of Systems Medicine, Tor Vergata University, Rome, Italy

    Diego Centonze

  4. Unit of Neurology, IRCCS Neuromed, Pozzilli, IS, Italy

    Diego Centonze

Authors
  1. Alessandra Musella
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  2. Diego Centonze
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Correspondence to Diego Centonze .

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Editors and Affiliations

  1. Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, L’Aquila, Italy

    Mauro Maccarrone

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Musella, A., Centonze, D. (2023). Electrophysiology of Endocannabinoid Signaling. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 2576. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2728-0_38

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  • DOI: https://doi.org/10.1007/978-1-0716-2728-0_38

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2727-3

  • Online ISBN: 978-1-0716-2728-0

  • eBook Packages: Springer Protocols

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