Abstract
Ergosterol, also known as provitamin D2, is a member of the steroid family. It is mainly found in some plants and mushrooms and is chemically related to cholesterol. As a major sterol in fungi, it acts as a general elicitor in plants and causes changes in membrane potential, production of reactive oxygen species, and modification of H+ fluxes and sometimes causes expression of phytoalexins. This chapter describes the extraction, purification, and analysis of ergosterol from Agaricus bisporus. The method discussed here includes extraction of the lipid fraction with n-hexane, followed by saponification. The final residue was eluted with methanol and analyzed using HPLC system. The second method involves extraction with methanol/dichloromethane (75:25, v/v), and the residue was dissolved in methanol and analyzed using an HPLC system. The third method uses extraction with chloroform/methanol mixture (20:10, v/v), and the analysis was done using HPLC. These methods can help in the potential use of mushrooms as an abundant source of ergosterol.
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Kottayi, M., Abhyankar, K. (2022). Ergosterol. In: Amin, D., Amaresan, N., Ray, S. (eds) Biotic Elicitors. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2601-6_16
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DOI: https://doi.org/10.1007/978-1-0716-2601-6_16
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