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A Primer for Circadian Metabolic Profile Analysis Using Multi-platform Metabolomics

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Circadian Clocks

Part of the book series: Neuromethods ((NM,volume 186))

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Abstract

Metabolomics is defined as the large-scale analysis of metabolites within a biological system. While small molecules, such as melatonin and cortisol, have been known to fluctuate in a circadian fashion for some time, recent studies have used metabolomics to expand the known cycling metabolome. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy are two primary analytical platforms readily utilized for metabolomics analyses. Here, we describe a set of metabolomics approaches for analysis of both polar metabolites and lipids, focusing on considerations for circadian studies. These include liquid chromatography and desorption electrospray ionization coupled to MS, NMR protocols, and complementary real-time bioluminescence tracing using firefly luciferase as a reporter of circadian gene expression. These methods can be used to study the impact of both central and peripheral clocks on metabolism from various biological samples.

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Acknowledgments

This work was supported by the National Institutes of Health (award numbers R01DK120757, R01NR018836, P01CA165997, and R01HL142981), National Institute of Environmental Health Sciences (award number T32ES01985, supporting L.N.B), and Pharmacology T32 Training Grant (award number T32 GM008076, supporting D.M.M).

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Author notes

  1. Ai Wen Tan, Dania M. Malik and Lisa N. Bottalico contributed equally.

Authors and Affiliations

  1. Department of Systems Pharmacology and Translational Therapeutics, Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Ai Wen Tan, Dania M. Malik, Lisa N. Bottalico, Arjun Sengupta & Aalim M. Weljie

  2. Pharmacology Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Dania M. Malik

  3. Center of Excellence in Environmental Toxicology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Lisa N. Bottalico & Aalim M. Weljie

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  1. Ai Wen Tan
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Correspondence to Aalim M. Weljie .

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Editors and Affiliations

  1. Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, Aichi, Japan

    Tsuyoshi Hirota

  2. Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, Aichi, Japan

    Megumi Hatori

  3. Regulatory Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA

    Satchidananda Panda

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Tan, A.W., Malik, D.M., Bottalico, L.N., Sengupta, A., Weljie, A.M. (2022). A Primer for Circadian Metabolic Profile Analysis Using Multi-platform Metabolomics. In: Hirota, T., Hatori, M., Panda, S. (eds) Circadian Clocks. Neuromethods, vol 186. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2577-4_16

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  • DOI: https://doi.org/10.1007/978-1-0716-2577-4_16

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  • Publisher Name: Humana, New York, NY

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