Abstract
In the preceding chapter, we introduced bioluminescence-optogenetics (BL-OG) and luminopsin fusion proteins (LMOs), an emerging method of molecular neuromodulation. In addition to reviewing the fundamental principles of BL-OG, we provided a discussion of its application in vitro, including with cell lines and primary cells in culture in vitro. BL-OG is mediated by an easily diffusible molecule, luciferin, and when applied systemically in rodents, the substrate can spread throughout the body, including the brain, achieving powerful molecular neuromodulation with convenience even in awake and behaving animals. In this chapter, we provide a practical guide for BL-OG and LMO applications in rodent models of the nervous system, both ex vivo and in vivo.
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Acknowledgments
Support for this project was provided by NSF CBET-1512826 (KB/REG), NIH F31NS115479 (MAS), R21NS112948 (REG), S10OD021773 (KB), DOD W81XWH1910776 (REG), and the Mirowski Family Foundation (REG).
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Stern, M.A., Skelton, H., Fernandez, A.M., Gutekunst, CA.N., Gross, R.E., Berglund, K. (2022). Applications of Bioluminescence-Optogenetics in Rodent Models. In: Kim, SB. (eds) Bioluminescence. Methods in Molecular Biology, vol 2525. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2473-9_27
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DOI: https://doi.org/10.1007/978-1-0716-2473-9_27
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