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Modeling Asthma in Mice Using Common Aeroallergens

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Asthma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2506))

Abstract

Aeroallergens are common inducers of asthma in humans and are widely used in experimental research to generate animal models of this disease. In this chapter, we describe four mouse models of aeroallergen-induced asthma. These models differ in type and number of allergens used, route and duration of allergen exposure, and utilization of an adjuvant, representing different mechanistic variants of asthma. In addition, we describe several basic methods that are commonly used in mechanistic studies of asthma in mice. These methods include tracheotomy and bronchoalveolar lavage, cytospin and morphologic analysis of bronchoalveolar lavage cells, and lung harvest and digestion for generation of single-cell suspension.

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Acknowledgment

This work was supported by the National Institutes of Health grants R01HL122995 and R01AI143837 to M.M.G.

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Authors and Affiliations

  1. Division of Allergy and Clinical Immunology, Department of Medicine, National Jewish Health, Denver, CO, USA

    Bidisha Paul Chowdhury & Magdalena M. Gorska

  2. Division of Allergy and Clinical Immunology, Department of Medicine, University of Colorado, Anschutz Medical Campus, Aurora, CO, USA

    Magdalena M. Gorska

Authors
  1. Bidisha Paul Chowdhury
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  2. Magdalena M. Gorska
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Corresponding author

Correspondence to Magdalena M. Gorska .

Editor information

Editors and Affiliations

  1. Division of Allergy and Clinical Immunology, Department of Medicine, National Jewish Health, Denver, CO, USA

    Magdalena M. Gorska

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Paul Chowdhury, B., Gorska, M.M. (2022). Modeling Asthma in Mice Using Common Aeroallergens. In: Gorska, M.M. (eds) Asthma. Methods in Molecular Biology, vol 2506. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2364-0_1

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  • DOI: https://doi.org/10.1007/978-1-0716-2364-0_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2363-3

  • Online ISBN: 978-1-0716-2364-0

  • eBook Packages: Springer Protocols

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