Abstract
Marine organisms have developed physiological and biochemical strategies to survive under the exposure of UV-B radiation. In particular, Antarctic marine bacteria, exposed to extremes of temperature, UV and ice, have adapted to cope with UV radiation by producing photoprotective molecules. Here, we describe (1) the sampling strategy to collect marine samples (surface water/ice and sediment samples) and (2) the selection strategy to isolate in these samples only UV-resistant marine bacteria.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Sheridan C (2012) Recasting natural product research. Nat Biotechnol 30:385–387
Williamson NR, Fineran PC, Leeper FJ et al (2006) The biosynthesis and regulation of bacterial prodiginines. Nat Rev Microbiol 4:887–899
Rehm BH (2010) Bacterial polymers: biosynthesis, modifications and applications. Nat Rev Microbiol 8:578–592
Jaspars M, De Pascale D, Andersen JH et al (2016) The marine biodiscovery pipeline and ocean medicines of tomorrow. J Mar Biol 96:151–158
Bruno S, Coppola D, di Prisco G et al (2019) Enzymes from marine polar regions and their biotechnological applications. Mar Drugs 17:544
Núñez-Pons L, Shilling A, Verde C et al (2020) Marine terpenoids from polar latitudes and their potential applications in biotechnology. Mar Drugs 18(8):401
Giordano D (2020) Bioactive molecules from extreme environments. Mar Drugs 18(12):640
Banaszak AT (2003) Photoprotective physiological and biochemical responses of aquatic organisms. In: Helbling EW, Zagarese H (eds) UV effects in aquatic organisms and ecosystems. Royal Society of Chemistry, Cambridge
Núñez-Pons L, Avila C, Romano G et al (2018) UV-protective compounds in marine organisms from the Southern Ocean. Mar Drugs 16(9):336
Correa-Llantén DN, Amenábar MJ, Blamey JM (2012) Antioxidant capacity of novel pigments from an Antarctic bacterium. J Microbiol 50:374–379
Órdenes-Aenishanslins N, Anziani-Ostuni G, Vargas-Reyes M et al (2016) Pigments from UV-resistant Antarctic bacteria as photosensitizers in dye sensitized solar cells. J Photochem Photobiol B 162:707–714
Silva TR, Tavares RSN, Canela-Garayoa R et al (2019) Chemical characterization and biotechnological applicability of pigments isolated from Antarctic bacteria. Mar Biotechnol 3:416–429
Silva TRE, Júnior SLCF, de Queiroz AC et al (2021) Pigments from Antarctic bacteria and their biotechnological applications. Crit Rev Biotechnol 23:1–28
Coppola D, Giordano D, Tinajero-Trejo M et al (2013) Antarctic bacterial hemoglobin and its role in the protection against nitrogen reactive species. Biochim Biophys Acta 1834:1923–1931
Joint I, Mühling M, Querellou J (2010) Culturing marine bacteria—an essential prerequisite for biodiscovery. Microb Biotechnol 3(5):564–575
Sipkema D, Schippers K, Maalcke WJ et al (2011) Multiple approaches to enhance the cultivability of bacteria associated with the marine sponge Haliclona (gellius) sp. Appl Environ Microbiol 77(6):2130–2140
Feller G, Gerday C (2003) Psychrophilic enzymes: hot topics in cold adaptation. Nat Rev Microbiol 1:200–208
Gerday C, Aittaleb M, Bentahir M et al (2000) Cold-adapted enzymes: from fundamentals to biotechnology. Trends Biotechnol 18(3):103–107
Schut F, de Vries EJ, Gottschal JC et al (1993) Isolation of typical marine bacteria by dilution culture: growth, maintenance, and characteristics of isolates under laboratory conditions. Appl Environ Microbiol 59(7):2150–2160
Acknowledgments
This research was financially supported by the Italian National Programme for Antarctic Research (PNRA) of the Ministry of University and Research (MUR), 2016/AZ1.06-Project PNRA16_00043. We wish to thank Giovanni Del Monaco and Francesca Segreti for technical support and assistance.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Coppola, D., Verde, C., Giordano, D. (2022). Isolation of UV-Resistant Marine Bacteria by UV-C Assays. In: Verde, C., Giordano, D. (eds) Marine Genomics. Methods in Molecular Biology, vol 2498. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2313-8_15
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2313-8_15
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2312-1
Online ISBN: 978-1-0716-2313-8
eBook Packages: Springer Protocols