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Strategies for Multienzyme Assemblies

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Multienzymatic Assemblies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2487))

Abstract

Proteins are not designed to be standalone entities and must coordinate their collective action for optimum performance. Nature has developed through evolution the ability to co-localize the functional partners of a cascade enzymatic reaction in order to ensure efficient exchange of intermediates. Inspired by these natural designs, synthetic scaffolds have been created to enhance the overall biological pathway performance. In this chapter, we describe several DNA- and protein-based scaffold approaches to assemble artificial enzyme cascades for a wide range of applications. We highlight the key benefits and drawbacks of these approaches to provide insights on how to choose the appropriate scaffold for different cascade systems.

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Acknowledgements

This review was supported by grants from NSF (CBE1803008, CBE1911950, MCB1817675, MCB2013991, and DMR1609621).

Author information

Author notes

  1. Qing Sun, Maryam Raeeszadeh-Sarmazdeh and Shen-Long Tsai contributed equally with all other contributors.

Authors and Affiliations

  1. Department of Chemical Engineering, Texas A&M University, College Station, TX, USA

    Qing Sun

  2. Department of Chemical and Materials Engineering, University of Nevada, Reno, NV, USA

    Maryam Raeeszadeh-Sarmazdeh

  3. Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei City, Taiwan

    Shen-Long Tsai

  4. Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA

    Wilfred Chen

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  1. Qing Sun
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  2. Maryam Raeeszadeh-Sarmazdeh
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  3. Shen-Long Tsai
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  4. Wilfred Chen
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Corresponding author

Correspondence to Wilfred Chen .

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Editors and Affiliations

  1. Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece

    Haralambos Stamatis

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Sun, Q., Raeeszadeh-Sarmazdeh, M., Tsai, SL., Chen, W. (2022). Strategies for Multienzyme Assemblies. In: Stamatis, H. (eds) Multienzymatic Assemblies. Methods in Molecular Biology, vol 2487. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2269-8_7

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  • DOI: https://doi.org/10.1007/978-1-0716-2269-8_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2268-1

  • Online ISBN: 978-1-0716-2269-8

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