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Molecular Farming in Seed Crops: Gene Transfer into Barley (Hordeum vulgare ) and Wheat (Triticum aestivum )

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Recombinant Proteins in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2480))

Abstract

The production of recombinant proteins in seed crops has a long history and cereal grains are now one of the platforms in commercial use. Specific advantages include excellent storage properties, a well-developed endomembrane system with a high biosynthetic capacity and well-established cultivation procedures worldwide. However, the production of transgenic cereals is a time-consuming procedure and the lack of efficient transformation systems is still a significant bottleneck. Barley can be transformed at high efficiency but the protocols are genotype-dependent. Wheat is generally more challenging to transform, but considerable progress has been made in enhancing transformation efficiencies and in controlling transgene expression. In this chapter, we describe and discuss standard procedures for generating transgenic barley and wheat for the production of recombinant proteins.

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Acknowledgments

The authors acknowledge funding of the project “Phenotyping Across Experimental Scales.”

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Authors and Affiliations

  1. Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria

    Eszter Kapusi & Eva Stoger

Authors
  1. Eszter Kapusi
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  2. Eva Stoger
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Corresponding author

Correspondence to Eva Stoger .

Editor information

Editors and Affiliations

  1. Fraunhofer IME, Aachen, Nordrhein-Westfalen, Germany

    Stefan Schillberg

  2. Fraunhofer IME, Aachen, Nordrhein-Westfalen, Germany

    Holger Spiegel

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kapusi, E., Stoger, E. (2022). Molecular Farming in Seed Crops: Gene Transfer into Barley (Hordeum vulgare ) and Wheat (Triticum aestivum ). In: Schillberg, S., Spiegel, H. (eds) Recombinant Proteins in Plants. Methods in Molecular Biology, vol 2480. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2241-4_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2241-4_3

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2240-7

  • Online ISBN: 978-1-0716-2241-4

  • eBook Packages: Springer Protocols

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