Abstract
Calcium Ca2+ regulation is a key component of numerous cellular functions. In cardiomyocytes, Ca2+ regulates excitation-contraction coupling and influences signaling cascades involved in cell metabolism and cell survival. Prolonged dysregulation of mitochondrial Ca2+ leads to dysfunctional cardiomyocytes, apoptosis and ultimately heart failure. VEGF promotes cardiomyocyte contractility by increasing calcium transients to control the strength of the heartbeat. Here, we describe a method to measure mitochondrial Ca2+ fluxes in human ventricular cardiomocytes after inducing stretch-mediated hypertrophy in vitro.
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Acknowledgments
M.L. Matter is supported by a grant from the National Institutes of Health (R01HD091162). C. Giorgi is supported by the Italian Association for Cancer Research (IG-19803) and the Progetti di Rilevante Interesse Nazionale (PRIN20177E9EPY). C. Giorgi is grateful for local funds from University of Ferrara and A-ROSE.
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Ramaccini, D., Giorgi, C., Matter, M.L. (2022). Measuring Mitochondrial Calcium Fluxes in Cardiomyocytes upon Mechanical Stretch-Induced Hypertrophy. In: Fiedler, L.R., Pellet-Many, C. (eds) VEGF Signaling. Methods in Molecular Biology, vol 2475. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2217-9_15
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DOI: https://doi.org/10.1007/978-1-0716-2217-9_15
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