Abstract
New methods to analyze cells and tissues in ambient condition without any harsh chemical fixation or physical freezing and drying are summarized in this report. The first approach, an atmospheric pressure mass spectrometry imaging method, is based on laser ablation in atmospheric pressure assisted by atmospheric plasma and nanomaterials such as nanoparticles and graphene to enhance laser ablation. The second one is based on secondary ion mass spectrometry (SIMS) imaging of live cells in solution capped with single-layer graphene to preserve intact and hydrated biological samples even under ultrahigh vacuum for SIMS bio-imaging in solution.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Passarelli MK, Pirkl A, Moellers R, Grinfeld D, Kollmer F, Havelund R, Newman CF, Marshall PS, Arlinghaus H, Alexander MR (2017) The 3D OrbiSIMS—label-free metabolic imaging with subcellular lateral resolution and high mass-resolving power. Nat Methods 14(12):1175–1183
Kompauer M, Heiles S, Spengler B (2017) Autofocusing MALDI mass spectrometry imaging of tissue sections and 3D chemical topography of nonflat surfaces. Nat Methods 14(12):1156–1158
Kourkoutis LF, Plitzko JM, Baumeister W (2012) Electron microscopy of biological materials at the nanometer scale. Annu Rev Mater Res 42:33–58
Svitkina T (2007) Electron microscopic analysis of the leading edge in migrating cells. Methods Cell Biol 79:295–319
Jungnickel H, Laux P, Luch A (2016) Time-of-flight secondary ion mass spectrometry (ToF-SIMS): a new tool for the analysis of toxicological effects on single cell level. Toxics 4(1):5
Kim JY, Seo ES, Kim H, Park J-W, Lim D-K, Moon DW (2017) Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution. Nat Commun 8(1):1–13
Kim JY, Seo ES, Lim HJ, Kim H, Park J-W, Shin HH, Lim D-K, Moon DW (2018) Nanomaterials and continuous wave laser-based efficient desorption for atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices. RSC Adv 8(15):8021–8025
Kim JY, Lee SY, Kim H, Park J-W, Lim D-K, Moon DW (2018) Biomolecular imaging of regeneration of zebrafish caudal fins using high spatial resolution ambient mass spectrometry. Anal Chem 90(21):12723–12730
Kim JY, Lim H, Lee SY, Song C, Park J-W, Shin HH, Lim D-K, Moon DW (2019) Graphene-coated glass substrate for continuous wave laser desorption and atmospheric pressure mass spectrometric imaging of a live hippocampal tissue. ACS Appl Mater Interfaces 11(30):27153–27161
Kim JY, Lee SY, Moon DW, Park J-W, Lim D-K, Song C (2019) Atmospheric pressure mass spectrometric imaging of bio-tissue specimen using electrospray-assisted CW laser desorption and ionization source. Biointerphases 14(4):041001
Lee SY, Lim H, Moon DW, Kim JY (2019) Improved ion imaging of slowly dried neurons and skin cells by graphene cover in time-of-flight secondary ion mass spectrometry. Biointerphases 14(5):051001
Lim H, Lee SY, Park Y, Jin H, Seo D, Jang YH, Moon DW (2021) Mass spectrometry imaging of untreated wet cell membranes in solution using single-layer graphene. Nat Methods 18:316. https://doi.org/10.1038/s41592-020-01055-6
Buskila Y, Breen PP, Tapson J, Van Schaik A, Barton M, Morley JW (2014) Extending the viability of acute brain slices. Sci Rep 4(1):1–7
Opitz-Araya X, Barria A (2011) Organotypic hippocampal slice cultures. J Vis Exp (48):2462
Lu X, Jiang Z, Xiong Q, Tang Z, Hu X, Pan Y (2008) An 11 cm long atmospheric pressure cold plasma plume for applications of plasma medicine. Appl Phys Lett 92(8):081502
Li Q, Li J-T, Zhu W-C, Zhu X-M, Pu Y-K (2009) Effects of gas flow rate on the length of atmospheric pressure nonequilibrium plasma jets. Appl Phys Lett 95(14):141502
Sands BL, Huang SK, Ganguly BN (2009) Current scaling in an atmospheric pressure capillary dielectric barrier discharge. Appl Phys Lett 95(5):051502
BioMap. http://www.maldi-msi.org
MSiReader. https://msireader.wordpress.ncsu.edu/
Xu Y-F, Lu W, Rabinowitz JD (2015) Avoiding misannotation of in-source fragmentation products as cellular metabolites in liquid chromatography–mass spectrometry-based metabolomics. Anal Chem 87(4):2273–2281
Acknowledgments
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2021R1C1C2010928 and 2021R1A2C1008704) and the BK21 FOUR project funded by the Ministry of Education, Korea (4199990113966).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Kim, J.Y., Lim, H., Moon, D.W. (2022). Ambient Mass Spectrometry Imaging of Small Molecules from Cells and Tissues. In: Lee, YJ. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 2437. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2030-4_3
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2030-4_3
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2029-8
Online ISBN: 978-1-0716-2030-4
eBook Packages: Springer Protocols