Abstract
Cancer stem cells (CSCs) are a small subpopulation of self-renewing cancer cells that are present within tumors. Calculating the frequency of tumor-initiating cells is important in the assessment of the number of CSCs present in a cell population. In this chapter, we present a protocol developed for quantification of CSCs from breast cancer tumors that can be adapted to CSCs from other types of tumors.
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Acknowledgments
This work was supported by the NIH/NCI grants (2R01CA155243; 1R01CA200970), the CPRIT grant (RP170172) awarded to S.A.M, and the NIH T32 grant (5T32CA18682) awarded to N.A.K.
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den Hollander, P., Joseph, R., Vasaikar, S., Kuburich, N.A., Deshmukh, A.P., Mani, S.A. (2022). Limiting Dilution Tumor Initiation Assay: An In Vivo Approach for the Study of Cancer Stem Cells. In: Kannan, N., Beer, P. (eds) Stem Cell Assays. Methods in Molecular Biology, vol 2429. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1979-7_38
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DOI: https://doi.org/10.1007/978-1-0716-1979-7_38
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1978-0
Online ISBN: 978-1-0716-1979-7
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