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Fmoc Solid Phase Peptide Synthesis of Oxytocin and Analogues

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Oxytocin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2384))

Abstract

Solid phase peptide synthesis is the most commonly used method for the production of peptides. In this chapter, we outline the standard operating procedures used in our laboratory to efficiently access oxytocin-like peptides. This includes detailed descriptions of equipment setup, reagent selection, peptide assembly on solid support, peptide side chain deprotection and cleavage from the solid support, oxidative folding, purification, and analysis.

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Acknowledgments

M. Muttenthaler was supported by the European Research Council under the European Union’s Horizon 2020 research and innovation program (714366), by the Australian Research Council (DP190101667), the Vienna Science and Technology Fund (WWTF; LS18-053) and by Cancer Australia and Cancer Council Queensland grants (1146504). T. Kremsmayr was supported by the Austrian Academy of Sciences through a DOC Fellowship (25139).

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Authors and Affiliations

  1. Faculty of Chemistry, Institute of Biological Chemistry, University of Vienna, Vienna, Austria

    Thomas Kremsmayr & Markus Muttenthaler

  2. Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia

    Markus Muttenthaler

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  1. Thomas Kremsmayr
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  2. Markus Muttenthaler
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Correspondence to Markus Muttenthaler .

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Editors and Affiliations

  1. School of Chemistry, The University of Sydney, Camperdown, NSW, Australia

    Eryn L. Werry

  2. Research School of Chemistry, Australian National University, Canberra, ACT, Australia

    Tristan A. Reekie

  3. School of Chemistry, The University of Sydney, Camperdown, NSW, Australia

    Michael Kassiou

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Kremsmayr, T., Muttenthaler, M. (2022). Fmoc Solid Phase Peptide Synthesis of Oxytocin and Analogues. In: Werry, E.L., Reekie, T.A., Kassiou, M. (eds) Oxytocin. Methods in Molecular Biology, vol 2384. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1759-5_11

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  • DOI: https://doi.org/10.1007/978-1-0716-1759-5_11

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