Abstract
Eggplant is one of the five important, worldwide-distributed solanaceous crops. The use of anther culture technology to produce pure, 100% homozygous doubled haploid lines for hybrid seed production is possible since 1982, where the first protocol of wide application to different eggplant materials was published. From then on, different improvements and adaptations to different materials have been made. In parallel, protocols to implement isolated microspore culture technology in eggplant have been developed principally in the last decade, which opens the door for a more efficient DH production in this species. In this chapter, two protocols, one for anther and other for isolated microspore culture in eggplant, are described. Some steps and materials are common to both approaches. A detailed description of each step from is provided.
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Acknowledgments
This work was supported by grants AGL2017-88135-R and PID2020-115763RB-I00 to JMSS from Spanish MICINN jointly funded by FEDER. PC-M is a recipient of a contract of the “Juan de la Cierva” program of the Spanish Government. ACS is a recipient of a FPU predoctoral fellowship of the Spanish Ministry of Education.
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Calabuig-Serna, A., Porcel, R., Corral-Martínez, P., Seguí-Simarro, J.M. (2021). Anther and Isolated Microspore Culture in Eggplant (Solanum melongena L.). In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2288. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1335-1_14
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DOI: https://doi.org/10.1007/978-1-0716-1335-1_14
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