Abstract
Leek (A. ampeloprasum L.) is an economically important vegetable crop from Alliaceae family. It is a non–bulb forming biennial species grown for its pseudostem and leaves. Leek is a tetraploid with one of the largest genomes known among cultivated plant species. It has enormous economic importance all around the world for many purposes such as vegetable, medicinal herb, and food seasoning. Production and consumption of leek is in rise all around the world and breeders are trying to develop new F1 hybrid varieties with desired agronomical traits. Although self-compatible, leek shows high tendency toward outcrossing and display severe inbreeding depression when selfed with its own pollen. Therefore, inbred development through classical breeding techniques is very difficult in this crop. Traditional leek genotypes are highly heterozygous, open pollinated varieties. There is a high demand for F1 hybrid varieties with resistance to biotic and abiotic stresses and high-quality plants. Our group is trying to incorporate gynogenesis-based doubled haploid technology to leek improvement programs. Over the years, many experiments were carried out to determine the gynogenic potential of donor leek genotypes of different genetic backgrounds in different induction media. Here, we report a protocol allowing production of green gynogenic leek plants via single step culture of unopened flower buds. Ploidy levels of gynogenic regenerants are determined by flow cytometry analysis. A majority of the gynogenic leek regenerants produced survived well in vivo.
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References
van der Meer QP, Hanelt P (1990) Leek (Allium ampeloprasum var. porrum). In: Brewster JL, Rabinowitch HD (eds) Onions and allied crops, Vol. III. Biochemistry, food science, and minor crops. CRC Press, Boca Raton, FL, pp 179–196
Alan AR, Celebi-Toprak F, Kaska A (2016) Production and evaluation of gynogenic leek (Allium ampeloprasum L.) plants. Plant Cell Tissue Organ Cult 125:249–259
Berninger E, Buret P (1967) Etude des deficients chlorophylliens chez deux especes cultivees du genre Allium: I’oignon A. cepa L. et le A. porrum. Ann Amel Plant 17:175–194
De Clercq H, Van Bockstaele E (2002) Leek: advances in agronomy and breeding. In: Rabinowitch HD, Currah L (eds) Allium crop science: recent advances. CABI Publishing, Wallingford, UK, pp 431–438
Block E (2010) Garlic and other Alliums: the lore and the science. Royal Society of Chemistry, Cambridge
De Wilde-Duyfjes B (1976) A revision of the genus Allium L. (Liliaceae) in Africa. Meded Landbouwhogeschool Wageningen, Netherlands, pp 76–311
Stearn WT (1978) European species of Allium and allied genera of Alliaceae: a synonymic enumeration. Annal Musei Goulandris 4:83–198
Jones HA, Mann LK (1963) Onion and their allies: botany, cultivation and utilization. Leonard Hill, London
Vavilov NI (1926) Studies on the origins of cultivated plants. Bull Appl Bot Plant Breed 16:1–245
FAO (2019) Food and Agriculture Organization of the United Nations. http://www.fao.org/faostat/en/#data/QC. Accessed 10 August 2019
Alfonso C, Ernesto F, Virginia L, Silvana M (1998) Porric acids a−C new antifungal dibenzofurans from the bulbs of Allium porrum L. Eur J Org Chem 4:661–663
Ayumi U, Jun O, Hitomi K et al (2009) Mechanisms of sulfide components expression and structural determination of substrate precursor in jumbo leek (Allium ampeloprasum L.). Nippon Shokuhin Kagaku Kogaku Kaishi 56(5):280–285
Ben Arfa A, Najjaa H, Yahia B et al (2015) Antioxidant capacity and phenolic composition as a function of genetic diversity of wild Tunisian leek (Allium ampeloprasum L.). Acad J Biotech 3:15–26
Griffiths G, Trueman L, Crowther T et al (2002) Onions as global benefit to health. Phytother Res 16:603–615
Hertog MGL, Hollman PCH, Katan MB (1992) Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J Agric Food Chem 40:2379–2383
Ozgur M, Akpinar-Bayaziy A, Ozcan T, Afolayan AJ (2011) Effect of dehydration on several physic-chemical properties and the antioxidant activity of leeks (Allium porrum L.). Not Bot Hort Agrobot Cluj-Napoca 39:144–151
Radovanović B, Mladenović J, Radovanović A et al (2015) Phenolic composition, antioxidant, antimicrobial and cytotoxic activities of Allium porrum L. (Serbia) extracts. J Food Nutr Res 3:564–569
Shon MY, Choi SD, Kahng GG et al (2004) Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onions. Food Chem Toxicol 42:659–666
De Clercq H, Peusens D, Roldán-Ruiz I, Van Bockstaele E (2003) Causal relationships between inbreeding, seed characteristics and plant performance in leek (Allium porrum L.). Euphytica 134:103–115
Schweisguth B (1970) Etudes preliminiares a lamelioration du poireau A porrum L. Proposition dune methode demaliration. Ann Amel Plant 20:215–231
Silvertand B (1996) Induction, maintenance and utilization of male sterility in leek (Allium ampeloprasum L.) PhD thesis, Department of Plant Breeding, Agricultural, Wageningen, Netherlands
Smith BM, Crowther TC (1995) Inbreeding depression and single cross hybrids in leek (Allium ampeloprasum ssp. porrum). Euphytica 86:87–94
Gray D, Steckel JRA (1986) Self-and open-pollination as factors influencing seed quality in leek (Allium porrum). Ann Appl Biol 108:167–170
Gagnebin F, Bonnet JC (1979) Quelques considerations sur la culture et l’amelioration du poireau. Revue Hort Suisse 52:112–116
Kampe R (1980) Untersuchungen zum ausmass von hybrideffekten bei porree. Archiv Züchtungsforschung 10:123–131
Pink DAC (1993) Leek (Allium ampeloprasum L.). In: Kalloo G, Bergh BO (eds) Genetic improvement of vegetable crops. Pergamon Press, Oxford, New York, pp 29–34
Silvertand B, Jacobsen E, Mazereeuw J et al (1995) Efficient in vitro regeneration of leek (Allium ampeloprasum L.) via flower stalk segments. Plant Cell Rep 14:423–427
De Clercq H, Baert J, Van Bockstaele E (1999) Breeding potential of Belgian landraces of leek (Allium ampeloprasum L. var porrum). Euphytica 106:101–109
Alan AR, Kaska A, Celebi-Toprak F (2013) Edible Allium improvement via doubled haploidy technology. Curr Opin Biotech 24:42
Celebi-Toprak F, Alan AR (2016) Optimization of gynogenesis induction in leek (Allium ampeloprasum var. porrum). J Biotech 230:S30. https://doi.org/10.1016/j.jbiotec.2016.05.126
Kaska A, Celebi-Toprak F, Alan AR (2012) Gynogenesis induction in edible Alliums. J Biotech 161:18
Kaska A, Celebi Toprak F, Alan AR (2013) Gynogenesis induction in leek (Allium ampeloprasum L.) breeding materials. Curr Opin Biotech 24:42
Schum A, Mattiesch L, Timmann EM, Hofmann K (1993) Regeneration of dihaploids via gynogenesis in Allium-Porrum L. Gartenbauwissenschaft 58(5):227–232
Peterson R, Slovin JP, Chen C (2010) A simplified method for differential staining of aborted and non-aborted pollen grains. Int J Plant Biology 1:e13
Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Report 9(3):211–215
Dolezel J, Bartos J (2005) Plant DNA flow cytometry and estimation of genome size. Ann Bot 95:99–110
Alan AR, Brants A, Cobb E, Goldschmied PA, Mutschler MA, Earle ED (2004) Fecund gynogenic lines from onion (Allium cepa L.) breeding materials. Plant Sci 167:1055–1066
Acknowledgments
This research was supported by grant from The Scientific and Technological Research Council of Turkey (TUBITAK-TOVAG, Project No. 113O232). Special thanks to members of PAU BIYOM (Pamukkale University, Denizli, Turkey).
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Celebi-Toprak, F., Alan, A.R. (2021). In Vitro Gynogenesis in Leek (Allium ampeloprasum L.). In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1315-3_7
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DOI: https://doi.org/10.1007/978-1-0716-1315-3_7
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