Summary
A new simple, efficient and rapid in vitro method for mass clonal propagation of leek (Allium ampeloprasum L.) plants, using small (5 mm) flower stalk (peduncle) explants, was established. Adventitious shoots were produced from single subepidermal cells. A wide variation in the percentage of regenerating explants and number of regenerated shoots per explant between individual plants within one cultivar was observed. The concentration of the growth regulators 6-benzylaminopurine and α-naphthalene-acetic acid influenced the percentage of regenerating explants and the average number of regenerated shoots per explant. A combination of 10 mg.l−1 6-benzylaminopurine and 10 mg.l−1 α-naphthalene-acetic acid, resulted in a maximum percentage of regenerating explants and a high average number of regenerated shoots per explant. The percentage of regenerating explants and the average number of regenerated shoots per explant decreased with increasing flower stalk length (age). The basal explants gave both the highest percentage of regenerating explants and average number of regenerated shoots per explant. An average of 300 shoots per flower stalk was obtained for all plants, making this new in vitro method a powerful tool in hybrid leek breeding.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Baumunk-Wende E (1989) Gartenbauwissenschaft 54: 20–24
Buiteveld J, Van der Valk P, Jansen J, Creemers-Molenaar J, Colijn-Hooymans CM (1993) Plant Cell Rep 12: 431–434
Brown DCW, Thorpe TA (1986) In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, Vol III, Academic Press, Florida: 49–65
Debergh PC, Standaert-de Metsenaere S (1976) Sci Hort 5: 11–12
De Bruyn MH, Ferreira DI, Slabbert MM, Pretorius J (1992) Plant Cell Tiss Org Cult 31: 179–184
DeMason DA (1979) Bot Gaz (Chicago) 140: 51–66
Doré C (1988) Agronomie 8: 509–511
Doré C, Schweisguth B (1980) Réunion Eucarpia, Versailles, INRA: 54–57
Dunstan DI, Short KC (1979) Sci Hort 11: 37–43
Feder N, O'Brian TP (1968) Amer J Bot 55: 123–142
Halperin W (1986) In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, Vol III, Academic Press, Florida: 20–38
Koopman W, Steegmans HHM, Pierik RLM (1987) Bloembollencultuur 31: 18–20
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Novák FJ, Havel L (1981) Biol Plant 23: 266–269
Novák FJ, Havel L, Dolezel J (1986) In: Evans DA, Sharp WR, Ammirato PV (eds) Handbook of Plant Cell Culture, vol IV Macmillan New York: 419–456
Pierik RLM, Steegmans HHM, Sprenkels HHM, Bijlsma JM (1985) Bloembollencultuur 45: 8–10
Rauber M, Grunewaldt J (1988) Plant Cell Rep 7: 426–429
Thorpe TA, Patel KR (1986) In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, Vol I, Academic Press, Florida: 49–60
Van Doome LE, Stolte AJ, Van Harten AM (1988), 4th EUCARPIA Allium symposium 1988
Van der Valk P, Scholten OE, Verstappen RC, Dons JJ (1992) Plant Cell Tiss Org Cult 30: 181–191
Ziv M, Hertz N, Biran Y (1983) Isr J Bot 32: 1–9
Author information
Authors and Affiliations
Additional information
Communicated by H. Lörz
Rights and permissions
About this article
Cite this article
Silvertand, B., Jacobsen, E., Mazereeuw, J. et al. Efficient in vitro regeneration of leek (Allium ampeloprasum L.) via flower stalk segments. Plant Cell Reports 14, 423–427 (1995). https://doi.org/10.1007/BF00234047
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00234047