Abstract
Optical tweezers enable the isolation and mechanical manipulation of individual nucleoprotein complexes. Here, we describe how to use this technique to interrogate the mechanical properties of individual protein-DNA complexes and extract information about their overall structural organization.
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Acknowledgments
This work was supported by Spanish Ministry of Science, Innovation and Universities grants BFU2015-63714-R and PGC2018-099341-B-I00 to BI and MINECO/FEDER grants FIS2010-17440, FIS2015-67765-R, and RTI2018-095802-B-I00 to FJCG.
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Morin, J.A., Cerrón, F., Cao-García, F.J., Ibarra, B. (2021). Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-Binding Protein-DNA Complexes. In: Oliveira, M.T. (eds) Single Stranded DNA Binding Proteins. Methods in Molecular Biology, vol 2281. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1290-3_17
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DOI: https://doi.org/10.1007/978-1-0716-1290-3_17
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