Abstract
Expression of chimeric antigen receptors can redirect T cell specificity and allow for MHC-independent recognition of melanoma associated antigens. Retroviral transduction is used to express chimeric antigen receptor constructs in murine CD4+ and CD8+ T cells. Here we describe the production of retroviral supernatants and the activation, transduction, expansion, and selection of murine T cells expressing the chimeric-PD1 receptor. This protocol can be modified for any murine chimeric antigen receptor construct.
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Acknowledgments
This work was supported in part by Longwood University’s Faculty Research Grants and Department of Biological and Environmental Sciences and Virginia Academy of Science’s Mary Louise Olds Andrews Cancer Grant.
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Barber, A. (2021). Generation of Murine Chimeric Antigen Receptor T Cells for Adoptive T Cell Therapy for Melanoma. In: Hargadon, K.M. (eds) Melanoma. Methods in Molecular Biology, vol 2265. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1205-7_44
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DOI: https://doi.org/10.1007/978-1-0716-1205-7_44
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1204-0
Online ISBN: 978-1-0716-1205-7
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