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Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells

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Cryopreservation and Freeze-Drying Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2180))

Abstract

Adipose-derived stem cells (ASCs) reside in the stromal compartment of adipose tissue and can be easily harvested in large quantities through a clinically safe liposuction procedure. ASCs do not induce immunogenic reactions and rather exert immunosuppressive effects. Therefore, they can be used for both autologous and allogeneic transplantations. They hold great promise for cell-based therapies and tissue engineering. A prerequisite to the realization of this promise is the development of successful cryopreservation methods for ASCs. In this chapter, we describe a xeno-free- and chemically defined cryopreservation protocol, which can be used for various clinical applications of ASCs.

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Author information

Authors and Affiliations

  1. Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA

    Melany López & Ali Eroglu

  2. Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta University, Augusta, GA, USA

    Ali Eroglu

Authors
  1. Melany López
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  2. Ali Eroglu
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Corresponding author

Correspondence to Ali Eroglu .

Editor information

Editors and Affiliations

  1. Unit for Reproductive Medicine—Clinic for Horses, Biostabilization Laboratory—Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, University of Veterinary Medicine Hannover, Hannover, Germany, University of Veterinary Medicine Hannover, Hannover, Germany

    Willem F. Wolkers

  2. Unit for Reproductive Medicine—Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

    Harriëtte Oldenhof

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López, M., Eroglu, A. (2021). Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells. In: Wolkers, W.F., Oldenhof, H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 2180. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0783-1_28

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  • DOI: https://doi.org/10.1007/978-1-0716-0783-1_28

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0782-4

  • Online ISBN: 978-1-0716-0783-1

  • eBook Packages: Springer Protocols

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