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In Silico-Guided Sequence Modification of Epitopes in Cancer Vaccine Development

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Immunoinformatics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2131))

Abstract

Discovery of tumor antigenic epitopes is important for cancer vaccine development. Such epitopes can be designed and modified to become more antigenic and immunogenic in order to overcome immunosuppression towards the native tumor antigen. In silico-guided modification of epitope sequences allows predictive discrimination of those that may be potentially immunogenic. Therefore, only candidates predicted with high antigenicity will be selected, constructed, and tested in the lab. Here, we described the employment of in silico tools using a multiparametric approach to assess both potential T-cell epitopes (MHC class I/II binding) and B-cell epitopes (hydrophilicity, surface accessibility, antigenicity, and linear epitope). A scoring and ranking system based on these parameters was developed to shortlist potential mimotope candidates for further development as peptide cancer vaccines.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia

    Winfrey Pui Yee Hoo, Pui Yan Siak & Lionel L. A. In

Authors
  1. Winfrey Pui Yee Hoo
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  2. Pui Yan Siak
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  3. Lionel L. A. In
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Corresponding author

Correspondence to Lionel L. A. In .

Editor information

Editors and Affiliations

  1. Department of BioMedical Engineering, Medical College of Wisconsin, Milwaukee, WI, USA

    Namrata Tomar

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Hoo, W.P.Y., Siak, P.Y., In, L.L.A. (2020). In Silico-Guided Sequence Modification of Epitopes in Cancer Vaccine Development. In: Tomar, N. (eds) Immunoinformatics. Methods in Molecular Biology, vol 2131. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0389-5_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0389-5_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0388-8

  • Online ISBN: 978-1-0716-0389-5

  • eBook Packages: Springer Protocols

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