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Mass Spectrometry for Biomarkers Discovery in Esophageal Squamous Cell Carcinoma

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Esophageal Squamous Cell Carcinoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2129))

Abstract

Mass spectrometry-based proteomics analysis could categorize proteins and study their interactions in large scale in human cancers. By this method, many proteins are upregulated or downregulated in esophageal squamous cell carcinoma (ESCC) when compared to nonneoplastic esophageal mucosae. The method can also be used to identify novel, effective biomarkers for early diagnosis or predict prognosis of patients with ESCC. These changes are associated with different clinical and pathological parameters. Different biological matrices such as pathological tissue, body fluids, and cancer cell lines-based proteomics have widely been used. Herein, we described cell line-based label-free shotgun proteomics (in-solution tryptic digestion) to identify the protein biomarkers differently expressed in ESCC.

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Author information

Authors and Affiliations

  1. Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Queensland, Australia

    Farhadul Islam & Vinod Gopalan

  2. Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh

    Farhadul Islam

  3. Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, Queensland, Australia

    Alfred K. Lam

Authors
  1. Farhadul Islam
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  2. Vinod Gopalan
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  3. Alfred K. Lam
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Corresponding author

Correspondence to Alfred K. Lam .

Editor information

Editors and Affiliations

  1. Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, QLD, Australia

    Alfred K. Lam

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Islam, F., Gopalan, V., Lam, A.K. (2020). Mass Spectrometry for Biomarkers Discovery in Esophageal Squamous Cell Carcinoma. In: Lam, A. (eds) Esophageal Squamous Cell Carcinoma. Methods in Molecular Biology, vol 2129. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0377-2_19

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  • DOI: https://doi.org/10.1007/978-1-0716-0377-2_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0376-5

  • Online ISBN: 978-1-0716-0377-2

  • eBook Packages: Springer Protocols

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