Abstract
Differentiating human induced pluripotent stem cells (iPSCs) into multipotent mesenchymal stem/stromal cells (MSCs) offers a renewable source of therapeutically invaluable cells. However, the process of MSC derivation from iPSCs suffers from an undesirably low efficiency. In this chapter, we present an optimized procedure to produce MSCs from human iPSCs with a high efficiency. The protocol depends on the generation of embryoid bodies (EBs) and requires the treatment of EBs with transforming growth factor beta 1 (TGF-β1). The resulting MSCs can be purified based on the expression of CD73, CD105, and CD90 markers and expanded for multiple passages without losing their characteristics.
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Abbreviations
- DPBS:
-
Dulbecco’s phosphate buffered saline
- EB:
-
Embryoid body
- FACS:
-
Fluorescence-activated cell sorting
- FBS:
-
Fetal bovine serum
- iPSC:
-
Induced pluripotent stem cell
- MSC:
-
Mesenchymal stem/stromal cell
- TGF-β1:
-
Transforming growth factor beta 1
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Acknowledgments
We thank the Gates Frontiers Fund and the Ehlers Danlos Center of Excellence at the University of Colorado Anschutz Medical Campus.
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McGarvey, S.S., Ferreyros, M., Kogut, I., Bilousova, G. (2021). Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells. In: Turksen, K. (eds) Induced Pluripotent Stem Cells and Human Disease. Methods in Molecular Biology, vol 2549. Humana, New York, NY. https://doi.org/10.1007/7651_2021_383
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DOI: https://doi.org/10.1007/7651_2021_383
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