Abstract
The extracellular matrix (ECM) is an important extracellular microenvironmental factor that regulates stem cell differentiation. The ECM is remodeled according to stem cell differentiation progression to precisely regulate the differentiation. Thus, it is expected that the matrices mimicking native ECM surrounding differentiating cells at each differentiation stage provide a favorable microenvironment to promote stem cell differentiation. However, it is difficult to prepare matrices mimicking native ECM using chemical methods because the ECM has a complicated composition. The decellularization technique is useful to prepare such matrices. In this chapter, we described the protocol to prepare matrices mimicking native ECM surrounding cells that are differentiating from mesenchymal stem cells to either osteoblasts or adipocytes via stem cell differentiation culture and a detergent- and nuclease-based decellularization technique.
Similar content being viewed by others
References
Hynes RO (2009) The extracellular matrix: not just pretty fibrils. Science 326:1216–1219
Giancotti FG, Ruoslahti E (1999) Integrin signaling. Science 285:1028–1032
Manabe R, Tsutsui K, Yamada T, Kimura M, Nakano I, Shimono C, Sanzen N, Furutani Y, Fukuda T, Oguri Y, Shimamoto K, Kiyozumi D, Sato Y, Sado Y, Senoo H, Yamashina S, Fukuda S, Kawai J, Sugiura N, Kimata K, Hayashizaki Y, Sekiguchi K (2008) Transcriptome-based systematic identification of extracellular matrix proteins. Proc Natl Acad Sci U S A 105:12849–12854
Daley WP, Peters SB, Larsen M (2008) Extracellular matrix dynamics in development and regenerative medicine. J Cell Sci 121:255–264
Page-McCaw A, Ewald AJ, Werb Z (2007) Matrix metalloproteinases and the regulation of tissue remodelling. Nat Rev Mol Cell Biol 8:221–233
Ioachim E, Charchanti A, Briasoulis E, Karavasilis V, Tsanou H, Arvanitis DL, Agrantis NJ, Pavlidis N (2002) Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: their prognostic value and role in tumour invasion and progression. Eur J Cancer 38:2362–2370
Kwon S-Y, Chae SW, Wilczynski SP, Arain A, Carpenter PM (2012) Laminin 322 expression in breast carcinoma. Appl Immunohistochem Mol Morphol 20:159–164
Hoshiba T, Lu H, Kawazoe N, Chen G (2010) Decellularized matrices for tissue engineering. Expert Opin Biol Ther 10:1717–1728
Hoshiba T, Chen G, Endo C, Maruyama H, Wakui M, Nemoto E, Kawazoe N, Tanaka M (2016) Decellularized extracellular matrix (ECM) as an in vitro model to study the comprehensive roles of the ECM in stem cell differentiation. Stem Cells Int. Article ID 6397820 doi: 10.1155/2016/6397820
Kerever A, Schnack J, Vellinga D, Ichikawa N, Moon C, Arikawa-Hirawasa E, Efird JT, Mercier F (2007) Novel extracellular matrix structures in the neural stem cell niche capture the neurogenic factor fibroblast growth factor from the extracellular milieu. Stem Cells 25:2146–2157
Hoshiba T, Kawazoe N, Tateishi T, Chen G (2009) Development of stepwise osteogenesis-mimicking matrices for the regulation of mesenchymal stem cell functions. J Biol Chem 284:31164–31173
Hoshiba T, Kawazoe N, Tateishi T, Chen G (2010) Development of extracellular matrices mimicking stepwise adipogenesis of mesenchymal stem cells. Adv Mater 22:3042–3047
Hoshiba T, Kawazoe N, Chen G (2011) Mechanism of regulation of PPARG expression of mesenchymal stem cells by osteogenesis-mimicking extracellular matrices. Biosci Biotechnol Biochem 75:2099–2104
Hoshiba T, Kawazoe N, Chen G (2012) The balance of osteogenic and adipogenic differentiation in human mesenchymal stem cells by matrices that mimic stepwise tissue development. Biomaterials 33:2025–2031
Acknowledgments
This work was supported by a Grant-in-Aid for Young Scientists (A) (26702016) and a Grant-in-Aid for Challenging Exploratory Research (16K15209), funded by MEXT, Japan.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media New York
About this protocol
Cite this protocol
Hoshiba, T., Kawazoe, N., Chen, G. (2017). Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_62
Download citation
DOI: https://doi.org/10.1007/7651_2017_62
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7655-3
Online ISBN: 978-1-4939-7656-0
eBook Packages: Springer Protocols