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Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry

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Sphingosine-1-Phosphate

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1697))

Abstract

Lysophosphatidic acids and sphingosine-1-phosphate are bioactive lipids that regulate diverse cellular and physiological processes through actions that are largely mediated by cell surface receptors. The roles played by these lipids in multiple disease processes make the enzymes and receptors involved in their synthesis, inactivation, and signaling attractive targets for pharmacological therapies. In this chapter we describe methods for sensitive accurate quantitation of LPA and S1P levels in biological fluids using liquid chromatography-coupled electrospray ionization tandem mass spectrometry.

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Authors and Affiliations

  1. Gill Heart and Vascular Institute, University of Kentucky College of Medicine, Lexington Veterans Affairs Medical Center, Lexington, KY, USA

    Maria P. Kraemer, Suchismita Halder, Susan S. Smyth & Andrew J. Morris

Authors
  1. Maria P. Kraemer
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  2. Suchismita Halder
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  3. Susan S. Smyth
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  4. Andrew J. Morris
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Corresponding author

Correspondence to Andrew J. Morris .

Editor information

Editors and Affiliations

  1. Centre for Eye Research Australia, The University of Melbourne, East Melbourne, Victoria, Australia

    Alice Pébay

  2. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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Kraemer, M.P., Halder, S., Smyth, S.S., Morris, A.J. (2017). Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry. In: Pébay, A., Turksen, K. (eds) Sphingosine-1-Phosphate. Methods in Molecular Biology, vol 1697. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_55

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  • DOI: https://doi.org/10.1007/7651_2017_55

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7412-2

  • Online ISBN: 978-1-4939-7413-9

  • eBook Packages: Springer Protocols

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