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Stable Isotope Analysis in Archaeological Science and Mummy Studies

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The Handbook of Mummy Studies

Abstract

Isotope analysis using bone collagen, bone apatite, and tooth enamel is widely performed to reconstruct the histories of societies and the biographies of individuals around the world. In such analyses, researchers have used a variety of elements, selectively according to their specific investigative objectives. Carbon and nitrogen isotope ratios, for instance, are useful for the estimation of ancient diets; strontium, lead, oxygen, hydrogen, and sulfur isotope ratios can track migrations and locate birthplaces. Mummification often maintains soft tissues that, relative to hard tissues, contain isotopic information that is of a shorter turnover rate and, therefore, contributing key details to personal and social histories. In this chapter, we introduce several cases of isotopic analysis of mummy tissues involving various elements.

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References

  • Ambrose SH, DeNiro MJ (1986) Reconstruction of African human diet using bone-collagen carbon and nitrogen isotope ratios. Nature 319:321–324

    Google Scholar 

  • Aubert D, Probst A, Stille P et al (2002) Evidence of hydrological control of Sr behavior in stream water (Strengbach catchment, Vosges mountains, France). Appl Geochem 17:285–300

    Google Scholar 

  • Babraj JA, Cuthbertson DJR, Smith K et al (2005) Collagen synthesis in human musculoskeletal tissues and skin. Am J Physiol Endocrinol Metab 289:864–869

    Google Scholar 

  • Bentley RA (2006) Strontium isotopes from the earth to the archaeological skeleton: a review. J Archaeol Method Theory 13(3):135–187

    Google Scholar 

  • Bowen GJ (2010) Statistical and geostatistical mapping of precipitation water isotope ratios. In: West JB, Bowen GJ, Dawson TE, Tu KP (eds) Isoscapes: understanding movement, pattern, and process on earth through isotope mapping. Springer, Berlin, pp 139–160

    Google Scholar 

  • Bowen GJ, Wilkinson B (2002) Spatial distribution of δ18O in meteoric precipitation. Geology 30(4):315–318

    Google Scholar 

  • Buzon MR, Bowen GJ (2009) Oxygen and carbon isotope analysis of human tooth enamel from the New Kingdom site of Tombos in Nubia. Archaeometry 52(5):855–868

    Google Scholar 

  • Buzon MR, Simonetti A, Creaser RA (2007) Migration in the Nile Valley during the New Kingdom period: a preliminary strontium isotope study. J Archaeol Sci 34:1391–1401

    Google Scholar 

  • Chisholm BS, Nelson DE, Schwarcz HP (1982) Stable carbon isotope ratios as a measure of marine versus terrestrial protein in ancient diets. Science 216:1131–1132

    Google Scholar 

  • Dansgaard W (1964) Stable isotopes in precipitation. Tellus 16:436–468

    Google Scholar 

  • DeNiro MJ, Epstein S (1978) Influence of diet on the distribution of carbon isotopes in animals. Geochim Cosmochim Acta 42:495–506

    Google Scholar 

  • DeNiro MJ, Epstein S (1981) Influence of diet on the distribution of nitrogen isotopes in animals. Geochim Cosmochim Acta 45:341–351

    Google Scholar 

  • Dufour E, Goepfert N, Gutierrez Leon B et al (2014) Pastoralism in northern Peru during pre-Hispanic times: insights from the Mochica period (100-800 AD) based on stable isotopic analysis of domestic camelids. PLoS One 9(1):e87559. https://doi.org/10.1371/journal.pone.0087559

    Article  Google Scholar 

  • Ehleringer JR, Bowen GJ, Chesson LA et al (2008) Hydrogen and oxygen isotope ratios in human hair are related to geography. PNAS 105(8):2788–2793

    Google Scholar 

  • Ezzo JA, Johnson CM, Price TD (1997) Analytical perspectives on prehistoric migration: a case study from east-Central Arizona. J Archaeol Sci 244:447–466

    Google Scholar 

  • Faure G (1977) Principles of isotope geology. Wiley, New York

    Google Scholar 

  • Fernández J, Panarello HO, Schobinger J (1999) The Inca mummy from Mount Aconcagua: decoding the geographic origin of the “messenger to the deities” by means of stable carbon, nitrogen, and sulfur isotope analysis. Geoarchaeology 14:27–46

    Google Scholar 

  • Finucane BC (2007) Mummies, maize, and manure: multi-tissue stable isotope analysis of late prehistoric human remains from the Ayacucho valley, Peru. J Archaeol Sci 34:2115–2124

    Google Scholar 

  • Francis PW, Moorbath S, Thorpe RS (1977) Strontium isotope data for recent andesites in Ecuador and North Chile. Earth Planet Sci Lett 37(2):197–202

    Google Scholar 

  • Fraser RA, Bogaard A, Heaton T et al (2011) Manuring and stable nitrogen isotope ratios in cereals and pulses: towards a new archaeobotanical approach to the inference of land use and dietary practices. J Archaeol Sci 38:2790–2804

    Google Scholar 

  • Grove MJ, Baker PA, Cross SL et al (2003) Application of strontium isotopes to understanding the hydrology and paleohydrology of the Altiplano, Bolivia–Peru. Palaeogeogr Palaeoclimatol Palaeoecol 194(1-3):281–297

    Google Scholar 

  • Handley LL, Austin AT, Robinson D et al (1999) The N-15 natural abundance (delta N-15) of ecosystem samples reflects measures of water availability. Aust J Plant Physiol 26(2):185–199

    Google Scholar 

  • Hawkesworth CJ, Hammill M, Gledhill AR et al (1982) Isotope and trace element evidence for late-stage intra-crustal melting in the the high Andes. Earth Planet Sci Lett 58:240–254

    Google Scholar 

  • Hedges REM, Clement JG, Thomas CDL et al (2007) Collagen turnover in the adult femoral mid-shaft: modeled from anthropogenic radiocarbon tracer measurements. Am J Phys Anthropol 133:808–816

    Google Scholar 

  • Hilson S (ed) (1996) Dental anthropology. Cambridge University Press, Cambridge

    Google Scholar 

  • Hoefs J (ed) (2004) Stable isotope geochemistry, 5th edn. Springer, Berlin

    Google Scholar 

  • Hoogewerff J, Papesch W, Kralik M et al (2001) The last domicile of the iceman from Hauslabjoch: a geochemical approach using Sr, C and O isotopes and trace element signatures. J Archaeol Sci 28:983–989

    Google Scholar 

  • Iacumin P, Bocherens H, Mariotti A et al (1996) An isotopic palaeoenvironmental study of human skeletal remains from the Nile Valley. Palaeogeogr Palaeoclimatol Palaeoecol 126:15–30

    Google Scholar 

  • Kamenov GD (2008) High-precision Pb isotopic measurements of teeth and environmental samples from Sofia (Bulgaria): insights for regional lead sources and possible pathways to the human body. Environ Geol 55:669–680

    Google Scholar 

  • Knudson KJ, Buikstra JE (2007) Residential mobility and resource use in the Chiribaya polity of southern Peru: strontium isotope analysis of archaeological tooth enamel and bone. Int J Osteoarchaeol 17(6):563–580

    Google Scholar 

  • Knudson KJ, Aufderheide AE, Buikstra JE (2007) Seasonality and paleodiet in the Chiribaya polity of southern Peru. J Archaeol Sci 34:451–462

    Google Scholar 

  • Knudson KJ, Williams SR, Osborn R et al (2009) The geographic origins of Nasca trophy heads using strontium, oxygen, and carbon isotope data. J Anthropol Archaeol 28:244–257

    Google Scholar 

  • Knudson KJ, Williams HM, Buikstra JE et al (2010) Introducing 88/86Sr analysis in archaeology: a demonstration of the utility of strontium isotope fractionation in paleodietary studies. J Archaeol Sci 37:2352–2364

    Google Scholar 

  • Knudson KJ, Pestle WJ, Torres-Rouff C et al (2012) Assessing the life history of an Andean traveler through biogeochemistry: stable and radiogenic isotope analyses of archaeological human remains from northern Chile. Int J Osteoarchaeol 22(4):435–451

    Google Scholar 

  • Koch PL, Halliday AN, Walter LM et al (1992) Sr isotopic composition of hydroxyapatite from recent and fossil salmon: the record of lifetime migration and diagenesis. Earth Planet Sci Lett 108:277–287

    Google Scholar 

  • Kutschera W, Müller W (2003) “Isotope language” of the alpine iceman investigated with AMS and MS. Nucl Instrum Methods Phys Res Sect B 204:705–719

    Google Scholar 

  • Ma Y, Fuller BT, Wei D et al (2016) Isotopic perspectives (13C, 15N, 34S) of diet, social complexity, and animal husbandry during the proto-Shang period (ca. 2000–1600 BC) of China. Am J Phys Anthropol 160:433–445

    Google Scholar 

  • Macko SA, Engel MH, Andrusevich V et al (1999) Documenting the diet in ancient human populations through stable isotope analysis of hair. Philos Trans R Soc London Ser B 354(1379):65–75

    Google Scholar 

  • Mariotti A (1983) Atmospheric nitrogen is a reliable standard for natural 15N abundance measurements. Nature 202:685–687

    Google Scholar 

  • Minagawa M, Wada E (1984) Stepwise enrichment of 15N along food chains: further evidence and the relation between 15N and animal age. Geochim Cosmochim Acta 48:1135–1140

    Google Scholar 

  • Nehlich O (2015) The application of Sulphur isotope analyses in archaeological research: a review. Earth Sci Rev 142:1–17

    Google Scholar 

  • O’Connell TC, Hedges REM (1999) Investigations into the effect of diet on modern human hair isotopic values. Am J Phys Anthropol 108:409–425

    Google Scholar 

  • O’Leary MH (1981) Carbon isotope fractionation in plants. Phytochemistry 20(4):553–567

    Google Scholar 

  • O’Leary MH (1988) Carbon isotopes in photosynthesis. Bioscience 38(5):328–336

    Google Scholar 

  • Oelze VM, Koch JK, Kupke K et al (2012) Multi-isotopic analysis reveals individual mobility and diet at the early Iron age monumental tumulus of Magdalenenberg, Germany. Am J Phys Anthropol 148:406–421

    Google Scholar 

  • Peri PL, Ladd B, Pepper DA et al (2012) Carbon (13C) and nitrogen (15N) stable isotope composition in plant and soil in southern Patagonia’s native forests. Glob Chang Biol 18:311–321

    Google Scholar 

  • Peterson BJ, Fry B (1987) Stable isotopes in ecosystem studies. Annu Rev Ecol Syst 18:293–320

    Google Scholar 

  • Ree CE, Jenkins WJ, Monster J (1978) The Sulphur isotopic composition of ocean water sulphate. Geochim Cosmochim Acta 42:377–381

    Google Scholar 

  • Richards MP, Fuller BT, Sponheimer M et al (2003) Sulphur isotopes in palaeodietary studies: a review and results from a controlled feeding experiment. Int J Osteoarchaol 13:37–45

    Google Scholar 

  • Sandford MK, Kissling GE (1993) Chemical analyses of human hair: anthropological applications. In: Sandford MK (ed) Investigations of ancient human tissue - chemical Anakysis in anthropology. Gordon and Breach, Langhorne, pp 131–166

    Google Scholar 

  • Schoeninger MJ, DeNiro MJ (1984) Nitrogen and carbon isotopic composition of bone collagen from marine and terrestrial animals. Geochim Cosmochim Acta 48:625–539

    Google Scholar 

  • Schoeninger MJ, DeNiro MJ, Tauber H (1983) Stable nitrogen isotope ratios of bone collagen reflect marine and terrestrial components of prehistoric human diet. Science 220:1381–1383

    Google Scholar 

  • Sillen A, Hall G, Richardson S et al (1998) 87Sr/86Sr ratios in modern and fossil food-webs of the Sterkfontein valley: implications for early hominid habitat preference. Geochim Cosmochim Acta 62(14):2463–2472

    Google Scholar 

  • Slovak NM, Paytan A, Wiegand BA (2009) Reconstructing middle horizon mobility patterns on the coast of Peru through strontium isotope analysis. J Archaeol Sci 36:157–165

    Google Scholar 

  • Smith BN, Epstein S (1971) Two categories of 13C/12C ratios for higher plants. Plant Physiol 47:380–384

    Google Scholar 

  • Szpak P, Millaire JF, White CD et al (2012) Influence of seabird guano and camelid dung fertilization on the nitrogen isotopic composition of field-grown maize (Zea mays). J Archaeol Sci 39:3721–3740

    Google Scholar 

  • Tomczak PD (2003) Prehistoric diet and socioeconomic relationships within the Osmore valley of southern Peru. J Anthropol Archaeol 22:262–278

    Google Scholar 

  • Touzeau A, Amiot R, Blichert-Toft J et al (2014) Diet of ancient Egyptians inferred from stable isotope systematics. J Archaeol Sci 46:114–124

    Google Scholar 

  • Turner BL, Kamenov GD, Kingston JD et al (2009) Insights into immigration and social class at Machu Picchu, Peru based on oxygen, strontium, and lead isotopic analysis. J Archaeol Sci 36(2):317–332

    Google Scholar 

  • White CD, Longstaffe FJ, Law KR (1999) Seasonal stability and variation in diet as reflected in human mummy tissues from the Kharga oasis and the Nile Valley. Palaeogeogr Palaeoclimatol Palaeoecol 147:209–222

    Google Scholar 

  • Wilson AS, Taylor T, Ceruti MC et al (2007) Stable isotope and DNA evidence for ritual sequences in Inca child sacrifice. PNAS 104(42):16456–16461

    Google Scholar 

  • Yoneda M, Suzuki R, Shibata Y et al (2004) Isotopic evidence of inland-water fishing by a Jomon population excavated from the Boji site, Nagano, Japan. J Archaeol Sci 31:97–107

    Google Scholar 

  • Zlotkin MDSH (1985) Hair analysis: a useful tool or a waste of money? Int J Dermatol 24(3):161–164

    Google Scholar 

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Correspondence to Mai Takigami .

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Takigami, M., Yoneda, M. (2020). Stable Isotope Analysis in Archaeological Science and Mummy Studies. In: Shin, D.H., Bianucci, R. (eds) The Handbook of Mummy Studies. Springer, Singapore. https://doi.org/10.1007/978-981-15-1614-6_8-1

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  • DOI: https://doi.org/10.1007/978-981-15-1614-6_8-1

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