Abstract
Inertial microfluidics has been widely employed as an important sample pretreatment technique for the single-cell analysis because of the distinct advantages of label-free and external field-free operation, high-throughput processing, and simple channel structure. In this chapter, the recent advances on the application of spiral inertial microfluidics as the sample pretreatment tool for single-cell analysis will be systematically summarized. Firstly, the underlying physics of the spiral inertial microfluidics will be introduced for better understanding of the working principle. Secondly, the design guidelines will be provided for the beginners or the researchers from other disciplines to efficiently design the devices of their applications. Thirdly, the recent advances on the application of spiral inertial microfluidics will be reviewed. Finally, the future perspective on inertial microfluidics will be discussed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Abdulla A, Liu W, Gholamipour-Shirazi A, Sun J, Ding X (2018) High-throughput isolation of circulating tumor cells using cascaded inertial focusing microfluidic channel. Anal Chem 90:4397–4405. https://doi.org/10.1021/acs.analchem.7b04210
Amini H, Lee W, Di Carlo D (2014) Inertial microfluidic physics. Lab Chip 14:2739–2761. https://doi.org/10.1039/C4LC00128A
Asmolov ES (1999) The inertial lift on a spherical particle in a plane Poiseuille flow at large channel Reynolds number. J Fluid Mech 381:63–87. https://doi.org/10.1017/S0022112098003474
Asmolov ES, Dubov AL, Nizkaya TV, Harting J, Vinogradova OI (2018) Inertial focusing of finite-size particles in microchannels. J Fluid Mech 840:613–630. https://doi.org/10.1017/jfm.2018.95
Berger SA, Talbot L, Yao LS (1983) Flow in curved pipes. Annu Rev Fluid Mech 15:461–512. https://doi.org/10.1146/annurev.fl.15.010183.002333
Bhagat AAS, Kuntaegowdanahalli SS, Papautsky I (2008) Continuous particle separation in spiral microchannels using Dean flows and differential migration. Lab Chip 8:1906–1914. https://doi.org/10.1039/B807107A
Bhagat AAS, Kuntaegowdanahalli SS, Kaval N, Seliskar CJ, Papautsky I (2010) Inertial microfluidics for sheath-less high-throughput flow cytometry. Biomed Microdevices 12:187–195. https://doi.org/10.1007/s10544-009-9374-9
Burke JM, Zubajlo RE, Smela E, White IM (2014) High-throughput particle separation and concentration using spiral inertial filtration. Biomicrofluidics 8:024105. https://doi.org/10.1063/1.4870399
Chen Q, Li D, Malekanfard A, Cao Q, Lin J, Wang M, Han X, Xuan X (2018) Tunable, sheathless focusing of diamagnetic particles in ferrofluid microflows with a single set of overhead permanent magnets. Anal Chem 90:8600–8606. https://doi.org/10.1021/acs.analchem.8b01813
Chhabra RP (2006) Bubbles, drops, and particles in non-Newtonian fluids. CRC press, New York
Choi S, Song S, Choi C, Park J-K (2008) Sheathless focusing of microbeads and blood cells based on hydrophoresis. Small 4:634–641. https://doi.org/10.1002/smll.200700308
Clime L, Li K, Geissler M, Hoa XD, Robideau GP, Bilodeau GJ, Veres T (2016) Separation and concentration of Phytophthora ramorum sporangia by inertial focusing in curving microfluidic flows. Microfluid Nanofluid 21:5. https://doi.org/10.1007/s10404-016-1844-9
Cox RG, Brenner H (1968) The lateral migration of solid particles in Poiseuille flow – I theory. Chem Eng Sci 23:147–173. https://doi.org/10.1016/0009-2509(68)87059-9
D’Avino G, Maffettone PL (2015) Particle dynamics in viscoelastic liquids. J Non-Newtonian Fluid Mech 215:80–104. https://doi.org/10.1016/j.jnnfm.2014.09.014
Dean WR, Chapman S (1928) Fluid motion in a curved channel. Proc R Soc Lond Ser A 121:402–420. https://doi.org/10.1098/rspa.1928.0205
Di Carlo D (2009) Inertial microfluidics. Lab Chip 9:3038–3046. https://doi.org/10.1039/B912547G
Di Carlo D, Irimia D, Tompkins RG, Toner M (2007) Continuous inertial focusing, ordering, and separation of particles in microchannels. Proc Natl Acad Sci 104:18892. https://doi.org/10.1073/pnas.0704958104
Di Carlo D, Edd JF, Humphry KJ, Stone HA, Toner M (2009) Particle segregation and dynamics in confined flows. Phys Rev Lett 102:094503. https://doi.org/10.1103/PhysRevLett.102.094503
Geng Z, Ju Y, Wang Q, Wang W, Li Z (2013a) Multi-component continuous separation chip composed of micropillar arrays in a split-level spiral channel. RSC Adv 3:14798–14806. https://doi.org/10.1039/C3RA41906A
Geng Z, Ju Y, Wang W, Li Z (2013b) Continuous blood separation utilizing spiral filtration microchannel with gradually varied width and micro-pillar array. Sensors Actuators B Chem 180:122–129. https://doi.org/10.1016/j.snb.2012.06.064
Ghadami S, Kowsari-Esfahan R, Saidi MS, Firoozbakhsh K (2017) Spiral microchannel with stair-like cross section for size-based particle separation. Microfluid Nanofluid 21:115. https://doi.org/10.1007/s10404-017-1950-3
Guan G, Wu L, Bhagat AA, Li Z, Chen PCY, Chao S, Ong CJ, Han J (2013) Spiral microchannel with rectangular and trapezoidal cross-sections for size based particle separation. Sci Rep 3:1475. https://doi.org/10.1038/srep01475
Heikali D, Di Carlo D (2010) A niche for microfluidics in portable hematology analyzers. J Assoc Lab Autom 15:319–328. https://doi.org/10.1016/j.jala.2010.02.005
Ho BP, Leal LG (1974) Inertial migration of rigid spheres in two-dimensional unidirectional flows. J Fluid Mech 65:365–400. https://doi.org/10.1017/S0022112074001431
Hosic S, Murthy SK, Koppes AN (2016) Microfluidic sample preparation for single cell analysis. Anal Chem 88:354–380. https://doi.org/10.1021/acs.analchem.5b04077
Hou HW, Warkiani ME, Khoo BL, Li ZR, Soo RA, Tan DS-W, Lim W-T, Han J, Bhagat AAS, Lim CT (2013) Isolation and retrieval of circulating tumor cells using centrifugal forces. Sci Rep 3:1259. https://doi.org/10.1038/srep01259
Huang Q, Mao S, Khan M, Zhou L, Lin J-M (2018) Dean flow assisted cell ordering system for lipid profiling in single-cells using mass spectrometry. Chem Commun 54:2595–2598. https://doi.org/10.1039/C7CC09608A
Hur SC, Tse HTK, Di Carlo D (2010) Sheathless inertial cell ordering for extreme throughput flow cytometry. Lab Chip 10:274–280. https://doi.org/10.1039/B919495A
Hur SC, Henderson-MacLennan NK, McCabe ERB, Di Carlo D (2011) Deformability-based cell classification and enrichment using inertial microfluidics. Lab Chip 11:912–920. https://doi.org/10.1039/C0LC00595A
Jackson JM, Witek MA, Kamande JW, Soper SA (2017) Materials and microfluidics: enabling the efficient isolation and analysis of circulating tumour cells. Chem Soc Rev 46:4245–4280. https://doi.org/10.1039/C7CS00016B
James DF (2008) Boger Fluids. Annu Rev Fluid Mech 41:129–142. https://doi.org/10.1146/annurev.fluid.010908.165125
Kang K, Lee SS, Hyun K, Lee SJ, Kim JM (2013) DNA-based highly tunable particle focuser. Nat Commun 4:2567. https://doi.org/10.1038/ncomms3567
Karimi A, Yazdi S, Ardekani AM (2013) Hydrodynamic mechanisms of cell and particle trapping in microfluidics. Biomicrofluidics 7:021501. https://doi.org/10.1063/1.4799787
Kemna EWM, Schoeman RM, Wolbers F, Vermes I, Weitz DA, van den Berg A (2012) High-yield cell ordering and deterministic cell-in-droplet encapsulation using Dean flow in a curved microchannel. Lab Chip 12:2881–2887. https://doi.org/10.1039/C2LC00013J
Khojah R, Stoutamore R, Di Carlo D (2017) Size-tunable microvortex capture of rare cells. Lab Chip 17:2542–2549. https://doi.org/10.1039/C7LC00355B
Khoo BL, Warkiani ME, Tan DS-W, Bhagat AAS, Irwin D, Lau DP, Lim AST, Lim KH, Krisna SS, Lim W-T, Yap YS, Lee SC, Soo RA, Han J, Lim CT (2014) Clinical validation of an ultra high-throughput spiral microfluidics for the detection and enrichment of viable circulating tumor cells. PLoS One 9:e99409. https://doi.org/10.1371/journal.pone.0099409
Kim YJ, Ahn WS, Lee SS, Kim MJ (2012) Lateral migration and focusing of colloidal particles and DNA molecules under viscoelastic flow. Lab Chip 12:2807–2814. https://doi.org/10.1039/C2LC40147A
Kim TH, Yoon HJ, Stella P, Nagrath S (2014) Cascaded spiral microfluidic device for deterministic and high purity continuous separation of circulating tumor cells. Biomicrofluidics 8:064117. https://doi.org/10.1063/1.4903501
Kim D, Sonker M, Ros A (2019) Dielectrophoresis: from molecular to micrometer-scale analytes. Anal Chem 91:277–295. https://doi.org/10.1021/acs.analchem.8b05454
Kulasinghe A, Tran THP, Blick T, O’Byrne K, Thompson EW, Warkiani ME, Nelson C, Kenny L, Punyadeera C (2017) Enrichment of circulating head and neck tumour cells using spiral microfluidic technology. Sci Rep 7:42517. https://doi.org/10.1038/srep42517
Kuntaegowdanahalli SS, Bhagat AAS, Kumar G, Papautsky I (2009) Inertial microfluidics for continuous particle separation in spiral microchannels. Lab Chip 9:2973–2980. https://doi.org/10.1039/B908271A
Kwon T, Prentice H, Oliveira JD, Madziva N, Warkiani ME, Hamel J-FP, Han J (2017) Microfluidic cell retention device for perfusion of mammalian suspension culture. Sci Rep 7:6703. https://doi.org/10.1038/s41598-017-06949-8
Lee WC, Bhagat AAS, Huang S, Van Vliet KJ, Han J, Lim CT (2011) High-throughput cell cycle synchronization using inertial forces in spiral microchannels. Lab Chip 11:1359–1367. https://doi.org/10.1039/C0LC00579G
Lee LM, Rosano Jenna M, Wang Y, Klarmann GJ, Garson CJ, Prabhakarpandian B, Pant K, Alvarez LM, Lai E (2018a) Label-free mesenchymal stem cell enrichment from bone marrow samples by inertial microfluidics. Anal Methods 10:713–721. https://doi.org/10.1039/C7AY02500A
Lee Y, Guan G, Bhagat AA (2018b) ClearCell® FX, a label-free microfluidics technology for enrichment of viable circulating tumor cells. Cytometry A 93:1251–1254. https://doi.org/10.1002/cyto.a.23507
Leshansky AM, Bransky A, Korin N, Dinnar U (2007) Tunable nonlinear viscoelastic “focusing” in a microfluidic device. Phys Rev Lett 98:234501. https://doi.org/10.1103/PhysRevLett.98.234501
Lim EJ, Ober TJ, Edd JF, Desai SP, Neal D, Bong KW, Doyle PS, McKinley GH, Toner M (2014) Inertio-elastic focusing of bioparticles in microchannels at high throughput. Nat Commun 5:4120. https://doi.org/10.1038/ncomms5120
Liu C, Ding B, Xue C, Tian Y, Hu G, Sun J (2016a) Sheathless focusing and separation of diverse nanoparticles in viscoelastic solutions with minimized shear thinning. Anal Chem 88:12547–12553. https://doi.org/10.1021/acs.analchem.6b04564
Liu C, Xue C, Sun J, Hu G (2016b) A generalized formula for inertial lift on a sphere in microchannels. Lab Chip 16:884–892. https://doi.org/10.1039/C5LC01522G
Liu C, Guo J, Tian F, Yang N, Yan F, Ding Y, Wei J, Hu G, Nie G, Sun J (2017) Field-free isolation of exosomes from extracellular vesicles by microfluidic viscoelastic flows. ACS Nano 11:6968–6976. https://doi.org/10.1021/acsnano.7b02277
Lu X, Xuan X (2015) Continuous microfluidic particle separation via elasto-inertial pinched flow fractionation. Anal Chem 87:6389–6396. https://doi.org/10.1021/acs.analchem.5b01432
Lu X, Liu C, Hu G, Xuan X (2017) Particle manipulations in non-Newtonian microfluidics: a review. J Colloid Interface Sci 500:182–201. https://doi.org/10.1016/j.jcis.2017.04.019
MacDonald MP, Spalding GC, Dholakia K (2003) Microfluidic sorting in an optical lattice. Nature 426:421–424. https://doi.org/10.1038/nature02144
Martel JM, Toner M (2012) Inertial focusing dynamics in spiral microchannels. Phys Fluids 24:032001. https://doi.org/10.1063/1.3681228
Martel JM, Toner M (2013) Particle focusing in curved microfluidic channels. Sci Rep 3:3340. https://doi.org/10.1038/srep03340
Matas J-P, Morris JF, Guazzelli É (2004) Inertial migration of rigid spherical particles in Poiseuille flow. J Fluid Mech 515:171–195. https://doi.org/10.1017/S0022112004000254
McGrath J, Jimenez M, Bridle H (2014) Deterministic lateral displacement for particle separation: a review. Lab Chip 14:4139–4158. https://doi.org/10.1039/C4LC00939H
Miller B, Jimenez M, Bridle H (2016) Cascading and parallelising curvilinear inertial focusing systems for high volume, wide size distribution, separation and concentration of particles. Sci Rep 6:36386. https://doi.org/10.1038/srep36386
Moon H-S, Je K, Min J-W, Park D, Han K-Y, Shin S-H, Park W-Y, Yoo CE, Kim S-H (2018) Inertial-ordering-assisted droplet microfluidics for high-throughput single-cell RNA-sequencing. Lab Chip 18:775–784. https://doi.org/10.1039/C7LC01284E
Nathamgari SSP, Dong B, Zhou F, Kang W, Giraldo-Vela JP, McGuire T, McNaughton RL, Sun C, Kessler JA, Espinosa HD (2015) Isolating single cells in a neurosphere assay using inertial microfluidics. Lab Chip 15:4591–4597. https://doi.org/10.1039/C5LC00805K
Nguyen N-T, Wu Z (2004) Micromixers – a review. J Micromech Microeng 15:R1–R16. https://doi.org/10.1088/0960-1317/15/2/r01
Nilsson J, Evander M, Hammarström B, Laurell T (2009) Review of cell and particle trapping in microfluidic systems. Anal Chim Acta 649:141–157. https://doi.org/10.1016/j.aca.2009.07.017
Nivedita N, Papautsky I (2013) Continuous separation of blood cells in spiral microfluidic devices. Biomicrofluidics 7:054101. https://doi.org/10.1063/1.4819275
Nivedita N, Ligrani P, Papautsky I (2017) Dean flow dynamics in low-aspect ratio spiral microchannels. Sci Rep 7:44072. https://doi.org/10.1038/srep44072
Oomen PE, Aref MA, Kaya I, Phan NTN, Ewing AG (2019) Chemical analysis of single cells. Anal Chem 91:588–621. https://doi.org/10.1021/acs.analchem.8b04732
Petchakup C, Tay HM, Yeap WH, Dalan R, Wong SC, Li KHH, Hou HW (2018) Label-free leukocyte sorting and impedance-based profiling for diabetes testing. Biosens Bioelectron 118:195–203. https://doi.org/10.1016/j.bios.2018.07.052
Plaks V, Koopman CD, Werb Z (2013) Circulating tumor cells. Science 341:1186–1188. https://doi.org/10.1126/science.1235226
Rafeie M, Zhang J, Asadnia M, Li W, Warkiani ME (2016) Multiplexing slanted spiral microchannels for ultra-fast blood plasma separation. Lab Chip 16:2791–2802. https://doi.org/10.1039/C6LC00713A
Ramachandraiah H, Svahn HA, Russom A (2017) Inertial microfluidics combined with selective cell lysis for high throughput separation of nucleated cells from whole blood. RSC Adv 7:29505–29514. https://doi.org/10.1039/C7RA02992F
Rodd LE, Cooper-White JJ, Boger DV, McKinley GH (2007) Role of the elasticity number in the entry flow of dilute polymer solutions in micro-fabricated contraction geometries. J Non-Newtonian Fluid Mech 143:170–191. https://doi.org/10.1016/j.jnnfm.2007.02.006
Romeo G, D’Avino G, Greco F, Netti PA, Maffettone PL (2013) Viscoelastic flow-focusing in microchannels: scaling properties of the particle radial distributions. Lab Chip 13:2802–2807. https://doi.org/10.1039/C3LC50257K
Ryu H, Choi K, Qu Y, Kwon T, Lee JS, Han J (2017) Patient-derived airway secretion dissociation technique to isolate and concentrate immune cells using closed-loop inertial microfluidics. Anal Chem 89:5549–5556. https://doi.org/10.1021/acs.analchem.7b00610
Saffman PG (1965) The lift on a small sphere in a slow shear flow. J Fluid Mech 22:385–400. https://doi.org/10.1017/S0022112065000824
Sajeesh P, Sen AK (2014) Particle separation and sorting in microfluidic devices: a review. Microfluid Nanofluid 17:1–52. https://doi.org/10.1007/s10404-013-1291-9
Schonberg JA, Hinch EJ (1989) Inertial migration of a sphere in Poiseuille flow. J Fluid Mech 203:517–524. https://doi.org/10.1017/S0022112089001564
Segre G, Silberberg A (1961) Radial particle displacements in Poiseuille flow of suspensions. Nature 189:209–210. https://doi.org/10.1038/189209a0
Seo J, Lean MH, Kole A (2007a) Membrane-free microfiltration by asymmetric inertial migration. Appl Phys Lett 91:033901. https://doi.org/10.1063/1.2756272
Seo J, Lean MH, Kole A (2007b) Membraneless microseparation by asymmetry in curvilinear laminar flows. J Chromatogr A 1162:126–131. https://doi.org/10.1016/j.chroma.2007.05.110
Seo KW, Kang YJ, Lee SJ (2014) Lateral migration and focusing of microspheres in a microchannel flow of viscoelastic fluids. Phys Fluids 26:063301. https://doi.org/10.1063/1.4882265
Shapiro HM (1983) Multistation multiparameter flow cytometry: a critical review and rationale. Cytometry 3:227–243. https://doi.org/10.1002/cyto.990030402
Shen S, Kou L, Zhang X, Wang D, Niu Y, Wang J (2018) Regulating secondary flow in ultra-low aspect ratio microchannels by dimensional confinement. Adv Theory Simul 1:1700034. https://doi.org/10.1002/adts.201700034
Sofela S, Sahloul S, Rafeie M, Kwon T, Han J, Warkiani ME, Song Y-A (2018) High-throughput sorting of eggs for synchronization of C. elegans in a microfluidic spiral chip. Lab Chip 18:679–687. https://doi.org/10.1039/C7LC00998D
Son J, Murphy K, Samuel R, Gale BK, Carrell DT, Hotaling JM (2015) Non-motile sperm cell separation using a spiral channel. Anal Methods 7:8041–8047. https://doi.org/10.1039/C5AY02205C
Son J, Samuel R, Gale BK, Carrell DT, Hotaling JM (2017) Separation of sperm cells from samples containing high concentrations of white blood cells using a spiral channel. Biomicrofluidics 11:054106. https://doi.org/10.1063/1.4994548
Song Y, Tian T, Shi Y, Liu W, Zou Y, Khajvand T, Wang S, Zhu Z, Yang C (2017) Enrichment and single-cell analysis of circulating tumor cells. Chem Sci 8:1736–1751. https://doi.org/10.1039/C6SC04671A
Squires TM, Quake SR (2005) Microfluidics: fluid physics at the nanoliter scale. Rev Mod Phys 77:977–1026. https://doi.org/10.1103/RevModPhys.77.977
Stoecklein D, Di Carlo D (2019) Nonlinear microfluidics. Anal Chem 91:296–314. https://doi.org/10.1021/acs.analchem.8b05042
Sun J, Li M, Liu C, Zhang Y, Liu D, Liu W, Hu G, Jiang X (2012) Double spiral microchannel for label-free tumor cell separation and enrichment. Lab Chip 12:3952–3960. https://doi.org/10.1039/C2LC40679A
Syed MS, Rafeie M, Vandamme D, Asadnia M, Henderson R, Taylor RA, Warkiani ME (2018) Selective separation of microalgae cells using inertial microfluidics. Bioresour Technol 252:91–99. https://doi.org/10.1016/j.biortech.2017.12.065
Tay HM, Yeap WH, Dalan R, Wong SC, Hou HW (2018) Multiplexed label-free fractionation of peripheral blood mononuclear cells for identification of monocyte–platelet aggregates. Anal Chem 90:14535–14542. https://doi.org/10.1021/acs.analchem.8b04415
Wang J, Lu W, Tang C, Liu Y, Sun J, Mu X, Zhang L, Dai B, Li X, Zhuo H, Jiang X (2015) Label-free isolation and mRNA detection of circulating tumor cells from patients with metastatic lung cancer for disease diagnosis and monitoring therapeutic efficacy. Anal Chem 87:11893–11900. https://doi.org/10.1021/acs.analchem.5b03484
Warkiani ME, Guan G, Luan KB, Lee WC, Bhagat AAS, Kant Chaudhuri P, Tan DS-W, Lim WT, Lee SC, Chen PCY, Lim CT, Han J (2014a) Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells. Lab Chip 14:128–137. https://doi.org/10.1039/C3LC50617G
Warkiani ME, Khoo BL, Tan DS-W, Bhagat AAS, Lim W-T, Yap YS, Lee SC, Soo RA, Han J, Lim CT (2014b) An ultra-high-throughput spiral microfluidic biochip for the enrichment of circulating tumor cells. Analyst 139:3245–3255. https://doi.org/10.1039/C4AN00355A
Warkiani ME, Tay AKP, Guan G, Han J (2015) Membrane-less microfiltration using inertial microfluidics. Sci Rep 5:11018. https://doi.org/10.1038/srep11018
Winter M, Hardy T, Rezaei M, Nguyen V, Zander-Fox D, Ebrahimi Warkiani M, Thierry B (2018) Isolation of circulating fetal trophoblasts using inertial microfluidics for noninvasive prenatal testing. Adv Mater Technol 3:1800066. https://doi.org/10.1002/admt.201800066
Wu L, Guan G, Hou HW, Bhagat AAS, Han J (2012) Separation of leukocytes from blood using spiral channel with trapezoid cross-section. Anal Chem 84:9324–9331. https://doi.org/10.1021/ac302085y
Xiang N, Ni Z (2015) High-throughput blood cell focusing and plasma isolation using spiral inertial microfluidic devices. Biomed Microdevices 17:110. https://doi.org/10.1007/s10544-015-0018-y
Xiang N, Chen K, Sun D, Wang S, Yi H, Ni Z (2013a) Quantitative characterization of the focusing process and dynamic behavior of differently sized microparticles in a spiral microchannel. Microfluid Nanofluid 14:89–99. https://doi.org/10.1007/s10404-012-1025-4
Xiang N, Yi H, Chen K, Sun D, Jiang D, Dai Q, Ni Z (2013b) High-throughput inertial particle focusing in a curved microchannel: insights into the flow-rate regulation mechanism and process model. Biomicrofluidics 7:044116. https://doi.org/10.1063/1.4818445
Xiang N, Chen K, Dai Q, Jiang D, Sun D, Ni Z (2015a) Inertia-induced focusing dynamics of microparticles throughout a curved microfluidic channel. Microfluid Nanofluid 18:29–39. https://doi.org/10.1007/s10404-014-1395-x
Xiang N, Shi Z, Tang W, Huang D, Zhang X, Ni Z (2015b) Improved understanding of particle migration modes in spiral inertial microfluidic devices. RSC Adv 5:77264–77273. https://doi.org/10.1039/C5RA13292D
Xiang N, Zhang X, Dai Q, Cheng J, Chen K, Ni Z (2016) Fundamentals of elasto-inertial particle focusing in curved microfluidic channels. Lab Chip 16:2626–2635. https://doi.org/10.1039/C6LC00376A
Xiang N, Ni Z, Yi H (2018a) Concentration-controlled particle focusing in spiral elasto-inertial microfluidic devices. Electrophoresis 39:417–424. https://doi.org/10.1002/elps.201700150
Xiang N, Shi X, Han Y, Shi Z, Jiang F, Ni Z (2018b) Inertial microfluidic syringe cell concentrator. Anal Chem 90:9515–9522. https://doi.org/10.1021/acs.analchem.8b02201
Xiang N, Han Y, Jia Y, Shi Z, Yi H, Ni Z (2019a) Flow stabilizer on a syringe tip for hand-powered microfluidic sample injection. Lab Chip 19:214–222. https://doi.org/10.1039/C8LC01051J
Xiang N, Wang J, Li Q, Han Y, Huang D, Ni Z (2019b) Precise size-based cell separation via the coupling of inertial microfluidics and deterministic lateral displacement. Anal Chem 91:10328. https://doi.org/10.1021/acs.analchem.9b02863
Xiang N, Zhang R, Han Y, Ni Z (2019c) A multilayer polymer-film inertial microfluidic device for high-throughput cell concentration. Anal Chem 91:5461–5468. https://doi.org/10.1021/acs.analchem.9b01116
Xuan X, Zhu J, Church C (2010) Particle focusing in microfluidic devices. Microfluid Nanofluid 9:1–16. https://doi.org/10.1007/s10404-010-0602-7
Yamada M, Seki M (2005) Hydrodynamic filtration for on-chip particle concentration and classification utilizing microfluidics. Lab Chip 5:1233–1239. https://doi.org/10.1039/B509386D
Yang S, Kim JY, Lee SJ, Lee SS, Kim JM (2011) Sheathless elasto-inertial particle focusing and continuous separation in a straight rectangular microchannel. Lab Chip 11:266–273. https://doi.org/10.1039/C0LC00102C
Yeo LY, Friend JR (2014) Surface acoustic wave microfluidics. Annu Rev Fluid Mech 46:379–406. https://doi.org/10.1146/annurev-fluid-010313-141418
Yin H, Marshall D (2012) Microfluidics for single cell analysis. Curr Opin Biotechnol 23:110–119. https://doi.org/10.1016/j.copbio.2011.11.002
Yousuff CM, Hamid NHB, Basha IHK, Ho ETW (2017) Output channel design for collecting closely-spaced particle streams from spiral inertial separation devices. AIP Adv 7:085004. https://doi.org/10.1063/1.4986617
Yuan D, Zhao Q, Yan S, Tang S-Y, Alici G, Zhang J, Li W (2018) Recent progress of particle migration in viscoelastic fluids. Lab Chip 18:551–567. https://doi.org/10.1039/C7LC01076A
Zhang J, Yan S, Yuan D, Alici G, Nguyen N-T, Ebrahimi Warkiani M, Li W (2016) Fundamentals and applications of inertial microfluidics: a review. Lab Chip 16:10–34. https://doi.org/10.1039/C5LC01159K
Zhang X, Zhu Z, Xiang N, Long F, Ni Z (2018) Automated microfluidic instrument for label-free and high-throughput cell separation. Anal Chem 90:4212–4220. https://doi.org/10.1021/acs.analchem.8b00539
Zheng S, Lin H, Liu J-Q, Balic M, Datar R, Cote RJ, Tai Y-C (2007) Membrane microfilter device for selective capture, electrolysis and genomic analysis of human circulating tumor cells. J Chromatogr A 1162:154–161. https://doi.org/10.1016/j.chroma.2007.05.064
Zhou J, Papautsky I (2013) Fundamentals of inertial focusing in microchannels. Lab Chip 13:1121–1132. https://doi.org/10.1039/C2LC41248A
Zhou Y, Ma Z, Tayebi M, Ai Y (2019) Submicron particle focusing and exosome sorting by wavy microchannel structures within viscoelastic fluids. Anal Chem 91:4577–4584. https://doi.org/10.1021/acs.analchem.8b05749
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 Springer Nature Singapore Pte Ltd, part of Springer Nature
About this entry
Cite this entry
Xiang, N., Ni, Z. (2022). Inertial Microfluidics for Single-Cell Manipulation and Analysis. In: Santra, T.S., Tseng, FG. (eds) Handbook of Single-Cell Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-10-8953-4_29
Download citation
DOI: https://doi.org/10.1007/978-981-10-8953-4_29
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-8952-7
Online ISBN: 978-981-10-8953-4
eBook Packages: EngineeringReference Module Computer Science and Engineering