Abstract
More than 20 different species of mollicutes, most of them belonging to the genus Mycoplasma, have been identified from ruminant hosts to date. While a considerable part of this group is conceived to be of minor epidemiological relevance, it contains some important pathogenic agents that have specific host ranges. Due to several peculiar properties of mycoplasmas, which include the absence of a cell wall and the capability of surface antigen variation, diseases caused by mycoplasmas are difficult to control in the conventional fashion by chemotherapy or immunoprophylaxis. Another general feature of mycoplasma infections is their protracted and occasionally chronic course. Five mycoplasmas of economic and welfare importance are mentioned here and serve as examples for the variety of clinical and diagnostic circumstances that can be resolved using polymerase chain reaction (PCR).
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References
Pfützner, H., and Sachse K. (1996) Mycoplasma bovis as an agent of mastitis, pneumonia, arthritis and genital disorders in cattle. Rev. Sci. Tech. 15, 1477–1494.
Bergonier, D., Berthelot, X., and Poumarat, F. (1997) Contagious agalactia of small ruminants: current knowledge concerning epidemiology, diagnosis and control. Rev. Sci. Tech. Off. Int. Epiz. 16, 848–873.
Nicholas, R. A., and Bashiruddin, J. B. (1995) Mycoplasma mycoides subspecies mycoides (small colony variant): the agent of contagious bovine pleuropneumonia and member of the "e;Mycoplasma mycoides cluster"e;. J. Comp. Pathol. 113, 1–27.
Rodriguez, J. L., Poveda, J. B., Oros, J., Herraez, P., Sierra M. A., and Fernandez, A. (1995) High mortality in goats associated with the isolation of a strain of Mycoplasma mycoides subsp. mycoides (large colony type). Zentralbl. Veterinärmed. B 42, 587–593.
Giacometti, M., Nicolet, J., Johansson, K.-E., Naglic, T., Degiorgis, M. P., and Frey, J. (1999) Detection and identification of Mycoplasma conjunctivae in infectious keratoconjunctivitis by PCR based on the 16S rRNA gene. Zentralbl. Veterinärmed. B 46, 173–180.
Johansson, K.-E., Heldtander, M. U., and Pettersson, B. ( 1998) Characterization of mycoplasmas by PCR and sequence analysis with universal 16S rDNA primers in Mycoplasma Protocols, (Miles, R. J., and Nicholas, R. A. J., ), Methods in Molecular Biology, vol. 104, Humana Press, Totowa, NJ, USA, pp. 145–165
Wirth, M., Berthold, E., Grashoff, M., Pfützner, H., Schubert, U., and Hauser, H. (1994) Detection of mycoplasma contaminations by the polymerase chain reaction. Cytotechnology 16, 67–77.
Hotzel, H. and Sachse, K. (1998) Improvement and acceleration of the diagnosis of contagious bovine pleuropneumonia by direct detection of the pathogen using the polymerase chain reaction. Berl. Münch. Tierärztl. Wochenschr. 111, 268–272.
Chávez González, Y. R., Ros Bascunana C., Bölske, G., Mattsson, J. G., Fernández Molina, C., and Johansson, K.-E. (1995) In vitro amplification of the 16S rRNA genes from Mycoplasma bovis and Mycoplasma agalactiae by PCR. Vet. Microbiol. 47, 183–190.
Bölske, G., Mattsson, J. G., Ros Bascunana, C., Bergström, K., Wesonga H., and Johansson, K.-E. (1996) Diagnosis of contagious caprine pleuropneumonia by detection and identification of Mycoplasma capricolum subsp. capripneumoniae by PCR and restriction enzyme analysis. J. Clin. Microbiol. 34, 785–791.
Kobayashi, H., Hirose, K., Worarach, A., et al. (1998) In vitro amplification of the 16S rRNA genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR. J. Vet. Med. Sci. 60, 1299–1303.
Persson, A., Pettersson. B., Bölske, G., and Johansson, K.-E. (1999) Diagnosis of contagious bovine pleuropneumonia by PCR-laser-induced fluorescence and PCR-restriction endonuclease analysis based on the 16S rRNA genes of Mycoplasma mycoides subsp. mycoides SC. J. Clin. Microbiol. 37, 3815–3821.
Harasawa, R. (1999) Genetic relationships among mycoplasmas based on the 16S-23S rRNA spacer sequence. Microbiol. Immunol. 43, 127–132.
Harasawa, R., Hotzel, H., and Sachse, K. (2000) Comparison of 16S-23S rRNA intergenic spacer regions among strains of the Mycoplasma mycoides cluster and reassessment of the taxonomic position of Mycoplasma bovine group 7. Int. J. Syst. Evol. Microbiol. 50, 1325–1329.
Scheinert, P., Krausse, R., Ullmann, U., Söller, R., and Krupp, G. (1996) Molecular differentiation of bacteria by PCR amplification of the 16S-23S rRNA spacer. J. Microbiol. Meth. 26, 103–117.
Subramaniam, S., Bergonier, D., Poumarat, F., et al. (1998) Species identification of Mycoplasma bovis and Mycoplasma agalactiae based on the uvrC genes by PCR. Mol. Cell. Probes. 12, 161–169.
Hotzel, H., Sachse, K., and Pfützner, H. (1996) Rapid detection of Mycoplasma bovis in milk samples and nasal swabs using the polymerase chain reaction. J. Appl. Bacteriol. 80, 505–510.
Pinnow, C. C., Butler, J. A., Sachse, K., Hotzel, H., Timms, L. L., and Rosenbusch, R. F. (2001) Detection of Mycoplasma bovis in preservative-treated field milk samples. J. Dairy Sci. 84, 1640–1645.
Miserez, R., Pilloud, P., Cheng, X., Nicolet, J., Griot, C., and Frey, J. (1997) Development of a sensitive nested PCR method for the specific detection of Mycoplasma mycoides subsp. mycoides SC. Mol. Cell. Probes 11, 103–111.
Monnerat, M.-P., Thiaucourt, F., Poveda, J. B., Nicolet, J., and Frey, J. (1999) Genetic and serological analysis of lipoprotein LppA in Mycoplasma mycoides subsp. mycoides LC and Mycoplasma mycoides subsp. capri. Clin. Diagn. Lab. Immunol. 6, 224–230.
Vilei, E.M., Nicolet, J. and Frey, J. (1999) IS1634, a novel insertion element creating long, variable-length direct repeats, which is specific for Mycoplasma mycoides subsp. mycoides SC small-colony type. J. Bacteriol. 181, 1319–1323.
Sachse, K., Diller, R., and Hotzel. H. (2002) Analysis of a signature region in the 23S ribosomal RNA gene of Mycoplasma and Acholeplasma spp., a potential target region for identification and differentiation at class, genus, cluster, and species levels. Syst. Appl. Microbiol., in press.
Hotzel, H., Sachse, K., and Pfützner, H. (1996) A PCR scheme for differentiation of organisms belonging to the Mycoplasma mycoides cluster. Vet. Microbiol. 49, 31–43.
Hotzel, H., Heller, M., and Sachse, K. (1999) Enhancement of Mycoplasma bovis detection in milk samples by antigen capture prior to PCR. Mol. Cell. Probes 13, 175–178.
Bashiruddin, J. B., Taylor, T. K., and Gould, A. G. (1994) A PCR-based test for the specific identification of Mycoplasma mycoides subspecies mycoides SC. J. Vet. Diagn. Invest. 6, 428–434.
Dedieu, L., Mady, V. and Lefèvre, P. C. (1994) Development of a selective polymerase chain reaction assay for the detection of Mycoplasma mycoides subsp. mycoides SC (contagious bovine pleuropneumonia agent). Vet. Microbiol. 42, 327–339.
Rodriguez, J. L., Ermel, R. W., Kenny, T. P., Brooks, D. L., and DaMassa, A. J. (1997) Polymerase chain reaction and endonuclease digestion of selected members of the "e;Mycoplasma mycoides cluster"e; and Mycoplasma putrefaciens. J. Vet. Diagn. Invest. 9, 186–190.
Nicholas, R., Bashiruddin, J., Ayling, R., and Miles, R. (2000) Contagious bovine pleuropneumonia-a review of recent developments. Vet. Bull. 70, 827–838.
Naglic, T., Hotzel, H., Ball, H. J., Seol, B., and Busch, K. ( 2001) Studies on the aetiology of caprine mycoplasmosis in Croatia, in Mycoplasmas of Ruminants: Pathogenicity, Diagnostics, Epidemiology and Molecular Genetics (Poveda, J. B., Fernandez, A., Frey, J., and Johansson, K.-E., ) EUR 19693-COST Action 826, vol. 5, EC, Brussels, Belgium, pp. 137–140.
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Hotzel, H., Frey, J., Bashiruddin, J., Sachse, K. (2003). Detection and Differentiation of Ruminant Mycoplasmas. In: Sachse, K., Frey, J. (eds) PCR Detection of Microbial Pathogens. Methods in Molecular Biology™, vol 216. Humana Press. https://doi.org/10.1385/1-59259-344-5:231
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DOI: https://doi.org/10.1385/1-59259-344-5:231
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