Abstract
Many common molecular biology techniques including polymerase chain reaction (PCR) (1), Southern blotting (2), comparative genomic hybridization (3), and in situ hybridization (4) have been adapted for use with paraffin-embedded tissue (PET). PCR-amplified products from PET can be used for, among others, the analysis of loss of heterozygosity (1), gene amplification (5), direct sequencing, cloning, and characterization of genes (6).
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Akalu, A., Reichardt, J.K.V. (2002). Increasing PCR Sensitivity for Amplification from Paraffin-Embedded Tissues. In: Chen, BY., Janes, H.W. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 192. Humana Press. https://doi.org/10.1385/1-59259-177-9:067
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DOI: https://doi.org/10.1385/1-59259-177-9:067
Publisher Name: Humana Press
Print ISBN: 978-0-89603-969-8
Online ISBN: 978-1-59259-177-0
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