Abstract
The polymerase chain reaction (PCR) allows the rapid detection of infectious viruses or other microorganisms as well as the cause of genetic defects. By performing sequence analysis afterward, important additional information on the PCR products is obtained. Often direct sequencing of the PCR products is preferred over the more time-consuming and expensive procedure of cloning prior to sequencing.
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References
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© 1996 Humana Press Inc., Totowa, NJ
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Petry, H., Bachmann, B., Lüke, W., Hunsmann, G. (1996). PCR Sequencing with the Aid of Detergents. In: Rapley, R. (eds) PCR Sequencing Protocols. Methods in Molecular Biology™, vol 65. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-344-9:105
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DOI: https://doi.org/10.1385/0-89603-344-9:105
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-344-3
Online ISBN: 978-1-59259-551-8
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