Abstract
Diploid strains of asci, placed on suitable medium, sporulate and form four haploid spores/ascus, which may be arranged as tetrahedra, diamonds, or linearly. Strains forming linear arrays of spores in the ascus are rare, dissection of the asci with the micromanipulator is difficult, so the method is now little used. Hawthorne (1) used it extensively in his early studies of yeast genetics. Generally, the yeast spores are considered unordered, and the ascus walls are dissolved enzymatically and the spores separated with a micromanipulator and grown to form colonies, which may consist of haploid or diploid cells, depending on whether or not the strain is heterothallic. The segregation of auxotrophic or other markers can then be determined, and map distances, in centimorgans, estimated. Allelic relationships can also be investigated.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Hawthorne, D. C. (1955) Genetics 40, 511.
Mortimer, R. K. and Hawthorne, D. C. (1969) Yeast Genetics, in The Yeasts. (Rose, A. H. and Harrison, J. S., eds.), Academic, New York, pp. 385–460.
Fowell, R. R. (1969) Sporulation and Hybridization of Yeasts, in The Yeasts. (Rose, A. H. and Harrison, J. S., eds.), Academic, New York, pp. 303–383.
Fink, G. R. (1986) Manual of Methods in Yeast Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Dawes, I. W., Wright, J. F., Vezinhet, F., and Ajam, N. (1980) Separation on urografin gradients of subpopulations from sporulating Saccharomyces cerevisiae cultures. J. Gen. Microbiol. 119, 165–171.
Sherman, F. and Wakem, P. (1991) “Mapping yeast glues,” in guide to yeast genetics and molecular biology. Meth. Enzymol. 194, 38–57.
Mortimer, R. K. and Schild, D. S. (1983) Genetic mapping in Saccharomyces cerevisiae, in The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance. (Strathern, J. N., Jones, E. W., and Broach, J. R., eds.), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 11–26.
Sherman, F. Fink, G. R., and Hicks, J. L. (1986) Laboratory Course Manual for Methods in Yeast Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Mortimer, R. K., Contopolou, C. R., and King, J. S. (1992) Genetic and physical maps of Saccharomyces cerevisiae. Yeast 8, 817–902.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Humana Press Inc.,Totowa, NJ
About this protocol
Cite this protocol
Spencer, J.F.T., Spencer, D.M. (1996). Meiotic Analysis. In: Evans, I.H. (eds) Yeast Protocols. Methods in Molecular Biology™, vol 53. Humana Press. https://doi.org/10.1385/0-89603-319-8:51
Download citation
DOI: https://doi.org/10.1385/0-89603-319-8:51
Publisher Name: Humana Press
Print ISBN: 978-0-89603-319-1
Online ISBN: 978-1-59259-540-2
eBook Packages: Springer Protocols